Kryomicrophotometry as a Method for Analyzing the Intracapillary HbO2 Saturation of Organs Under Different O2 Supply Conditions

  • W. A. Grunewald
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 75)


During the last years we developed a method for the determination of the HbO2 saturation in capillary blood. Until now, measurements of this important O2 supply parameter were not possible. The developed method is a microkryophotometrical one. This method includes
  1. 1)

    the rapid-kryo-fixation of the HbO2 saturation in the blood vessels of an organ sample,

  2. 2)

    the sectioning of the frozen samples,

  3. 3)

    the microphotometrical measurement of the hemoglobin absorption spectra in the transected vessels of the tissue sections.



Carotid Body Pressure Side Organ Sample Hemoglobin Solution Thermic Isolation 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • W. A. Grunewald
    • 1
    • 2
  • D. W. Lübbers
    • 1
    • 2
  1. 1.Abt. am Lehrstuhl für PhysiologieUniversität Regensburg84 RegensburgGermany
  2. 2.Max-Planck-Institut für Systemphysiologie46 DortmundGermany

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