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Kryomicrophotometry as a Method for Analyzing the Intracapillary HbO2 Saturation of Organs Under Different O2 Supply Conditions

  • W. A. Grunewald
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 75)

Abstract

During the last years we developed a method for the determination of the HbO2 saturation in capillary blood. Until now, measurements of this important O2 supply parameter were not possible. The developed method is a microkryophotometrical one. This method includes
  1. 1)

    the rapid-kryo-fixation of the HbO2 saturation in the blood vessels of an organ sample,

     
  2. 2)

    the sectioning of the frozen samples,

     
  3. 3)

    the microphotometrical measurement of the hemoglobin absorption spectra in the transected vessels of the tissue sections.

     

Keywords

Carotid Body Pressure Side Organ Sample Hemoglobin Solution Thermic Isolation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Grunewald, W. A.: Der Einfluß des deaquatisierten Hämoglobins auf die kryophotometriseh bestimmte HbO2-Sättigung. Pflügers Arch. 335, R 4 (1972)Google Scholar
  2. Grunewald, W. A., Lübbers, D. W.: Fixation of the O2-saturation of the hemoglobin in the capillary blood by rapid freezing. Pflügers Arch. 307, R 12 (1969)Google Scholar
  3. Grunewald, W. A., Lübbers, D. W.: Determination of intracapillary HbO -saturation with cryomicrophotometric method applied to the rabbit myocardium. Pflügers Arch. 353, 255 – 273 (1975)PubMedCrossRefGoogle Scholar
  4. Lübbers, D. W., Wodick, R.: The examination of multicomponent systems in biological materials by means of a rapid scanning photometer. Appl. Optics 8, 1055 – 1062 (1969)CrossRefGoogle Scholar
  5. Lübbers, D. W., Wodick, R.: Schnelle Photometrie komplizierter biochemischer Mehrkomponentensysteme. Z. Anal. Chem. 261, 271 – 280 (1972)CrossRefGoogle Scholar
  6. Weigelt, Ch., Schneider, S., Grunewald, W. A., Lübbers, D. W.: Determination of HbO2 gradients in thin layers — a method to quantify facilitated diffusion. Pflügers Arch. 339, R 4 (1973)Google Scholar
  7. Weigelt, Ch., Grunewald, W. A., Lübbers, D. W.: Influence of back pressure on the course of the HbO2 saturation gradient in HbO2-loaded millipore membranes. Pflügers Arch. 343, R 5 (1973)Google Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • W. A. Grunewald
    • 1
    • 2
  • D. W. Lübbers
    • 1
    • 2
  1. 1.Abt. am Lehrstuhl für PhysiologieUniversität Regensburg84 RegensburgGermany
  2. 2.Max-Planck-Institut für Systemphysiologie46 DortmundGermany

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