Pyrene Butyric Acid: A Non-Invasive Probe for In Situ Intracellular Oxygen Concentration
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 75)
Historically there have been two major problems facing researchers trying to measure the intracellular concentration of oxygen, [O2]i. The first is the cellular damage, however slight, concomitant with entry into cells by the (micro)electrodes used in Polarographic techniques. The second drawback is the actual consumption, again however slight, of oxygen by the Polarographic electrode. We could like to report at this time the successful application of pyrene butyric acid (PBA) as an alternative to the older methods as a quantitative tool for non-invasive, nonconsumptive measurement of [O2]i.
KeywordsNADH Oxidation Anoxic Period NADH Fluorescence Polarographic Electrode Liver Cell Suspension
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