Advertisement

Pyrene Butyric Acid: A Non-Invasive Probe for In Situ Intracellular Oxygen Concentration

  • Frans F. Jöbsis
  • Michael H. Mitnick
  • Thomas R. Snow
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 75)

Abstract

Historically there have been two major problems facing researchers trying to measure the intracellular concentration of oxygen, [O2]i. The first is the cellular damage, however slight, concomitant with entry into cells by the (micro)electrodes used in Polarographic techniques. The second drawback is the actual consumption, again however slight, of oxygen by the Polarographic electrode. We could like to report at this time the successful application of pyrene butyric acid (PBA) as an alternative to the older methods as a quantitative tool for non-invasive, nonconsumptive measurement of [O2]i.

Keywords

NADH Oxidation Anoxic Period NADH Fluorescence Polarographic Electrode Liver Cell Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chance, B. 1957. Techniques for the Assay of the Respiratory Enzymes. Methods in Enzymology 4:273.CrossRefGoogle Scholar
  2. Chance, B. and Williams, G.R. 1955. Respiratory Enzymes in Oxidative Phosphorylation I-III. J. Biol. Chem. 217, 383–427.PubMedGoogle Scholar
  3. Chapman, J.B. 1972. Fluorometric Studies of Oxidative Metabolism in Isolated Papillary Muscle of the Rabbit. J. Gen. Physiol. 59, 135–154.PubMedCrossRefGoogle Scholar
  4. Jobsis, F. and Stainsby, W.N. 1968. Oxidation of NADH during Contractions of Circulated Mammalian Skeletal Muscle. Respir. Physiol. 4, 292–300.PubMedCrossRefGoogle Scholar
  5. Knopp, J.A. and I.S. Longmuir. 1972. Intracellular Measurement of Oxygen by Quenching of Fluorescence of Pyrenebutyric acid. Biochim. Biophys Acta 279, 393–397.PubMedCrossRefGoogle Scholar
  6. Vaughn, W.M. and Weber, G. 1970. Oxygen Quenching of Pyrenebutyric Acid Fluorescence in Water. A Dynamic Probe of the Microenvironment. Biochemistry 9, 465–473.CrossRefGoogle Scholar
  7. Yang, C.C. and Legallais, V. 1954. A Rapid and Sensitive Recording Spectrophotometer for the Visible and Ultraviolet Region I. Description and Performance. Rev. Sci. Instr. 25, 801.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Frans F. Jöbsis
    • 1
  • Michael H. Mitnick
    • 1
  • Thomas R. Snow
    • 1
  1. 1.Department of Physiology and PharmacologyDuke University Medical CenterDurhamUSA

Personalised recommendations