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A New Approach to the Experimental Measurement of Mean Tissue PO2

  • R. E. Forster
  • C. W. Goodwin
  • N. Itada
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 75)

Abstract

We have developed a method for the estimation of the total amount of reversibly bound + dissolved O2 in a tissue based upon the differential exchanges of 16O2 and 18O2 between blood and the tissues through which it passes. While both isotopes enter the tissues and are metabolized to the same extent (the isotope effect is negligible), any O2 outside the capillaries will appear as a distribution or dilution pool to the labelled but not the unlabelled O2. This will cause a delay in the passage of a pulse of 18O2 through the tissue relative to an indicator. Steady state conditions are maintained so that there is no significant alteration in the total concentrations of O2 in blood or tissue.

Keywords

Hind Limb Indocyanine Green Autologous Blood Electromagnetic Flow Dilution Pool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Coburn, R.F. and L.B. Mayers. Myoglobin O2 tension determined from measurements of carboxymyoglobin in skeletal muscle. Am. J. Physiol. 220:66–74, 1974.Google Scholar
  2. 2.
    Kagen, E.J. Myoglobin. New York: Columbia University, p. 42 (1973).Google Scholar
  3. 3.
    Rossi-Fanelli, A. and E. Antonini. Studies on the oxygen and carbon monoxide equilibria of human myoglobin. Arch. Biochem. Biophys. 77:478–492, 1958.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • R. E. Forster
    • 1
  • C. W. Goodwin
    • 1
  • N. Itada
    • 1
  1. 1.Department of PhysiologyUniversity of PennsylvaniaPhiladelphiaUSA

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