A New Approach to the Experimental Measurement of Mean Tissue PO2
We have developed a method for the estimation of the total amount of reversibly bound + dissolved O2 in a tissue based upon the differential exchanges of 16O2 and 18O2 between blood and the tissues through which it passes. While both isotopes enter the tissues and are metabolized to the same extent (the isotope effect is negligible), any O2 outside the capillaries will appear as a distribution or dilution pool to the labelled but not the unlabelled O2. This will cause a delay in the passage of a pulse of 18O2 through the tissue relative to an indicator. Steady state conditions are maintained so that there is no significant alteration in the total concentrations of O2 in blood or tissue.
KeywordsHind Limb Indocyanine Green Autologous Blood Electromagnetic Flow Dilution Pool
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