The Effect of Decapitation on the Oxidation-Reduction State of NADH and ECoG in the Brain of the Awake Rat
The effects of complete ischemia on brain energy metabolism have been studied in various animal preparation (1,2,3,4). One of the approaches on studying those effects is to convert the brain into a closed system, by decapitation, and after fixation of the tissue to measure changes in various metabolites such as glucose, lactate, ATP and creatine phosphate. The freezing procedure avoids most of the postmortem changes of brain metabolites levels, but the events of the first few seconds after decapitation are obscure.
KeywordsPostmortem Change Brain Energy Metabolism Secondary Increase NADH Level Freezing Procedure
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