Measurement of Oxygenation and Deoxygenation of a Single Red Cell of Chicken Embryo by Means of a Microphotometer
In order to overcome difficulties inherent in the flow reaction apparatus so far used for kinetic studies of red cells, we have developed a technique of microphotometry. Since in a microphotometer a small light beam of about 10 u is transmitted through a single red cell, the reactions with O2 and CO can be measured by flushing a reacting gas onto the red cells. In the previous studies (1,2), the method of reaction measurement was elaborated and the CO reaction was determined in the red cells existing in the chorioallantoic capillary of chicken embryo. Since then, by using the same method and material, we have measured the oxygenation and deoxygenation rates and examined the velocity of combination and dissociation of O2 due to the Bohr effect.
KeywordsChicken Embryo Dissociation Curve Bohr Effect Oxygen Dissociation Curve Velocity Factor
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