The Iron-Sulfur Centers and the Function of Hydrogenase from Clostridium Pasteurianum
Hydrogenase has widespread occurrence among bacteria and algae. Its function allows the organisms to grow under some stringent conditions. In essence, activation of molecular hydrogen by hydrogenase (e.g., H2:NAD+ oxidoreductase EC 188.8.131.52; H2:ferricytochrome c3 oxidoreductase EC 184.108.40.206) enables the organisms to use H2 as the primary source of energy and/or reductant. Alternatively, production of H2 through hydrogenase (e.g., H2:ferredoxin oxidoreductase EC 220.127.116.11) permits the organism to use protons, perhaps the most available oxidant under anaerobic conditions, as the terminal electron acceptor to dispose of the excess electrons produced in oxidative metabolism. The latter is particularly important for obligate anaerobes such as Clostridium pasteurianum. A more detailed discussion on the physiological role of hydrogenase among microorganisms appeared recently (Mortenson and Chen, 1974).
KeywordsElectron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Paramagnetic Resonance Signal Methyl Viologen Hydrogenase Activity
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