The Iron-Sulfur Centers and the Function of Hydrogenase from Clostridium Pasteurianum

  • Jiann-Shin Chen
  • Leonard E. Mortenson
  • Graham Palmer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 74)


Hydrogenase has widespread occurrence among bacteria and algae. Its function allows the organisms to grow under some stringent conditions. In essence, activation of molecular hydrogen by hydrogenase (e.g., H2:NAD+ oxidoreductase EC; H2:ferricytochrome c3 oxidoreductase EC enables the organisms to use H2 as the primary source of energy and/or reductant. Alternatively, production of H2 through hydrogenase (e.g., H2:ferredoxin oxidoreductase EC permits the organism to use protons, perhaps the most available oxidant under anaerobic conditions, as the terminal electron acceptor to dispose of the excess electrons produced in oxidative metabolism. The latter is particularly important for obligate anaerobes such as Clostridium pasteurianum. A more detailed discussion on the physiological role of hydrogenase among microorganisms appeared recently (Mortenson and Chen, 1974).


Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Paramagnetic Resonance Signal Methyl Viologen Hydrogenase Activity 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Jiann-Shin Chen
    • 1
    • 2
  • Leonard E. Mortenson
    • 1
    • 2
  • Graham Palmer
    • 1
    • 2
  1. 1.Department of Biological SciencesPurdue UniversityWest LafayetteUSA
  2. 2.Department of BiochemistryRice UniversityHoustonUSA

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