Purification and Properties of Lung Lysyl Oxidase, A Copper-Enzyme

  • James J. Shieh
  • Kerry T. Yasunobu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 74)


Lysyl oxidase is an enzyme which converts peptidyl lysyl or hydroxyllysyl to peptidyl α-amino adipic or δ-hydroxy1-α-amino-adipic ε-semialdehyde. These so called allysine residues are the precursors of desmosine and isodesmosine which form cross links in elastin. The enzyme was detected for the first time in chick bone by Pinnell and Martin (1). At the present time Harris et al (2), Siegel et al. (3), Stassen (4) and Vidal et al (5) have reported the isolation of highly purified preparations of the enzyme from chick aorta, chick cartilage, embroyonic chick cartilage and bovine aorta, respectively. In the present investigation, highly purified preparation of bovine lung lysyl oxidase was isolated and some of its properties are described in this report.


Potassium Phosphate Buffer Ammonium Sulfate Lysyl Oxidase Tritiated Water Bovine Lung 
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  1. 1.
    Pinnell, S.R. and G.R. Martin. (1968) Proc. Nat. Acad. Sci. USA 61, 708.PubMedCrossRefGoogle Scholar
  2. 2.
    Harris, E.D., W.A. Gonnerman, J.E. Savage and B.L. O’Dell (1974) Biochim. Biophys. Acta 341, 332.PubMedCrossRefGoogle Scholar
  3. 3.
    Siegel, R.C., S.R. Pinnell and G.R. Martin (1970) Biochem.9, 4486.CrossRefGoogle Scholar
  4. 4.
    Stassen, F.L.H. (1975). Fed. Proc.34, 2717 (abstract).Google Scholar
  5. 5.
    Vidal, G.P., J.J. Shieh and K.T. Yasunobu (1975) Biochem. Blophys. Res. Commun. 64, 989.CrossRefGoogle Scholar
  6. 6.
    Cuatrecasas, P. and C.B. Anfinsen (1971) Methods in Enzymology Vol. XXII, p. 234, Academic Press.Google Scholar
  7. 7.
    David, B.J. (1964) Ann. N.Y. Acad. Sci.121, 404.Google Scholar
  8. 8.
    Weber, K. and M. Osborn (1969). J. Biol. Chem. 244, 4406.PubMedGoogle Scholar
  9. 9.
    Yamada, H., Yasunobu, K.T., Yamano, T., and Mason, H.S. (1963) Nature, 198, 1092.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • James J. Shieh
    • 1
  • Kerry T. Yasunobu
    • 1
  1. 1.Dept. of Biochem-Biophysics, School of MedicineUniversity of HawaiiHonoluluUSA

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