Studies on Bovine Adrenal Ferredoxin
Native and reconstituted adrenal ferredoxins have been obtained in crystalline form. The apoprotein was prepared by treatment of the native protein with trichloroacetic acid. When the apoprotein was incubated with ferrous ion, sulfide and 2-mercaptoethanol, the recovery of the reconstituted protein was considerably low. The reconstitution was greatly enhanced by the presence of 8 M urea. The reconstituted protein was indistinguishable from the native protein with respect to enzymic activity, spectral properties and iron content. The apparent contents of labile sulfide in both native and reconstituted proteins were about one mole per mole of protein when analyzed according to the original methylene blue method without alkaline zinc incubation. Extension of the alkaline zinc incubation over 2 hr resulted in release of two moles of labile sulfide per mole of protein.
KeywordsNative Protein Adrenal Cortex DEAE Cellulose Spirulina Platensis Cysteic Acid
Unable to display preview. Download preview PDF.
- 2.Kimura, T. and Suzuki, K. (1967) J. Biol. Chenu 242, 485.Google Scholar
- 6.Takemori, S., Sato, H., Gomi, T., Suhara, K. and Katagiri, M. Biochem. Biophys. Res. Commun. In press.Google Scholar
- 9.Rosenthal, O. and Narasimhulu, S. (1969) in Methods in Enzymology (Colowick, S. P. and Kaplan, N. O., eds), Vol. XV, pp. 604, Academic Press, New York.Google Scholar
- 14.Wada, K. and Matsubara, H., Unpublished work.Google Scholar
- 15.Suhara, K., Takemori, S., Katagiri, M., Wada, K., Kobayashi, H. and Matsubara, H. Anal. Biochem. In press.Google Scholar
- 16.Greenstein, J. P. and Winits, M. (1961) in Chemistry of the Amino Acids, Vol. III, pp. 1899, John Wiley and Sons, New York.Google Scholar