Purification and Properties of Cytochrome P-450 from Adrenocortical Mitochondria and its Interaction with Adrenodoxin
Cytochrome P-450s from mammalian sources have been purified to a homogeneous state by several groups, and highly purified cytochrome P-450 from bacterial origin has been obtained In a crystalline form. Among the mammalian P-450s, the one from adrenal mitochondria (Harding et al., 1964) is the easiest to solubilize and the hydroxylating system can be reconstituted with purified components.
KeywordsElectron Paramagnetic Resonance Cholesterol Oxidase Side Chain Cleavage Soret Region Adrenodoxin Reductase
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