Characterization of Purified Cytochrome P-450scc and P-45011β from Bovine Adrenocortical Mitochondria
In the adrenal cortex, NADPH-adrenal ferredoxin reductase (adrenodoxin reductase)-adrenal ferredoxin (adrenodoxin)-cytochrome P-450 system has been known to be associated with the mitochondrial steroid hydroxylase activities, viz., cholesterol side-chain cleavage and steroid 11β- and 18-hydroxylations. In order to elucidate the reaction mechanism of the hydroxylase system, it is important that each component is available in a pure form. As previously reported, adrenodoxin reductase (1) and adrenodoxin (2,3) have been obtained in a pure state. The latter has been crystallized. Details will be discussed in a separate chapter (4).
KeywordsSodium Cholate Mitochondrial Pellet Performic Acid Electron Transfer System Sedimentation Velocity Experiment
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