Evidence for Participation of NADH-Dependent Reductase in the Reaction of Benzoate 1,2-Dioxygenase (Benzoate Hydroxylase)

  • Hitoshi Fujisawa
  • Mutsuo Yamaguchi
  • Takashi Yamauchi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 74)


Benzoate 1,2-dioxygenase which has so far been referred to as benzoate hydroxylase catalyzes the double hydroxylation of benzoate, namely the incorporation of two hydroxyl groups into benzoate, resulting in the formation of l,2-dihydro-l,2-dihydroxybenzoate (DHB). Both oxygen atoms of the two hydroxyl groups introduced into the substrate are derived from the molecular oxygen (1,2).


Ammonium Sulfate Flavin Adenine Dinucleotide Anthranilic Acid Sodium Dithionite Flavin Adenine Dinucleotide 


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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Hitoshi Fujisawa
    • 1
  • Mutsuo Yamaguchi
    • 1
  • Takashi Yamauchi
    • 1
  1. 1.Department of BiochemistryAsahikawa Medical CollegeAsahikawaJapan

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