Evidence for Participation of NADH-Dependent Reductase in the Reaction of Benzoate 1,2-Dioxygenase (Benzoate Hydroxylase)
Benzoate 1,2-dioxygenase which has so far been referred to as benzoate hydroxylase catalyzes the double hydroxylation of benzoate, namely the incorporation of two hydroxyl groups into benzoate, resulting in the formation of l,2-dihydro-l,2-dihydroxybenzoate (DHB). Both oxygen atoms of the two hydroxyl groups introduced into the substrate are derived from the molecular oxygen (1,2).
KeywordsAmmonium Sulfate Flavin Adenine Dinucleotide Anthranilic Acid Sodium Dithionite Flavin Adenine Dinucleotide
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- 5.Kobayashi, S., and Hayaishi, O. (1970) in Methods in Enzymology (Kolowick, S.P., and Kaplan, N.O., Eds.) Vol. XVII, pp. 505–510, Academic Press, New York.Google Scholar
- 7.Hayaishi, O. (1974) in Molecular Mechanisms of Oxygen Activation (Hayaishi, O., Ed.) pp. 1–28, Academic Press, New York and London.Google Scholar