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Kinins pp 65-73 | Cite as

A Sensitive Kinin Liberating Assay for Kininogenase in Rat Urine, Isolated Glomeruli and Tubules of Rat Kidney

  • K. Mann
  • R. Geiger
  • E. Werle
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 70)

Abstract

There are several hints, that the kallikrein-kinin-system of the kidney is involved in physiological regulation mechanisms and in pathological processes (1, 2, 3, 4, 5, 6, 7). A prerequisite to evaluate the significance of the system, is the possibility of measuring kallikrein activities in tissues, where it is formed, excreted and inactivated. Recently Nustad and Pierce (8) showed, that the rat kidney synthesizes four kallikreins, which are released unchanged into urine. But the localisation of kallikreins in the nephron is not known. Moreover, there is no specific rat-urine kallikrein assay available, which is sensitive enough to measure enzyme activities in isolated structures of the kidney. This report will be focused on these points.

Keywords

Deoxycholic Acid Incubation Volume Urine Kallikrein International Congress Series Double Diffusion Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • K. Mann
    • 1
  • R. Geiger
    • 2
  • E. Werle
    • 2
  1. 1.I. Med. KlinikUniversität MunchenMunichGermany
  2. 2.Institut für Klinische Chemie und Klinische BiochemieUniversität MunchenMunichGermany

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