Kinins pp 65-73 | Cite as

A Sensitive Kinin Liberating Assay for Kininogenase in Rat Urine, Isolated Glomeruli and Tubules of Rat Kidney

  • K. Mann
  • R. Geiger
  • E. Werle
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 70)


There are several hints, that the kallikrein-kinin-system of the kidney is involved in physiological regulation mechanisms and in pathological processes (1, 2, 3, 4, 5, 6, 7). A prerequisite to evaluate the significance of the system, is the possibility of measuring kallikrein activities in tissues, where it is formed, excreted and inactivated. Recently Nustad and Pierce (8) showed, that the rat kidney synthesizes four kallikreins, which are released unchanged into urine. But the localisation of kallikreins in the nephron is not known. Moreover, there is no specific rat-urine kallikrein assay available, which is sensitive enough to measure enzyme activities in isolated structures of the kidney. This report will be focused on these points.


Deoxycholic Acid Incubation Volume Urine Kallikrein International Congress Series Double Diffusion Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Adetuyibi, A., and Mills, J.H. (1972) Lancet 2, 203.PubMedCrossRefGoogle Scholar
  2. 2.
    Geller, R.G., Margolius, H.S., Pisano, J.H., and Keiser, H.R. (1972) Circ. Res. 31, 857.PubMedGoogle Scholar
  3. 3.
    Croxatto, H.R., and San Martin, M. (1970) Experientia (Basel) 26, 1216.CrossRefGoogle Scholar
  4. 4.
    Marin-Grez, M., Cottone, P., and Carretero, O.A. (1972) Amer. J. Physiol. 223, 794.PubMedGoogle Scholar
  5. 5.
    Carretero, O.A., and Oza, N.B. (1973) Proc. of a Int. workshop conference Los Angeles, International congress series No. 302.Google Scholar
  6. 6.
    Scili, A.G., Carretero, O.A., Hampton, A., and Oza, N.B. (1975) Fed. Proc. 37, 378.Google Scholar
  7. 7.
    Frey, E., Kraut, H., and Werle, E. (1968) Ferdinand Enke Verlag, Stuttgart.Google Scholar
  8. 8.
    Nustad, K., Vaaje, K., and Pierce, J.V. (1975) Br. J. Pharmac. 53, 229.Google Scholar
  9. 9.
    Habal, F.M., and Movat, H.Z. (1972) Res. Comm. in Chem. Pathol. and Pharmacol. 4, 477.Google Scholar
  10. 10.
    Habal, F.M., Movat, H.Z., and Burrowes, C.E. (1974) Biochem. Pharmacol. 23, 2291.PubMedCrossRefGoogle Scholar
  11. 11.
    Spragg, J., and Austen, K.F. (1974) Biochem. Pharmacol. 23, 781.PubMedCrossRefGoogle Scholar
  12. 12.
    Guder, W., Wiesner, W., Stukowski, B., and Wieland, O. (1971) Hoppe-Seyler’s Z. Physiol. Chemie 352, 1319.CrossRefGoogle Scholar
  13. 13.
    Werle, E., and Vogel, R. (1960) Archs. int. Pharmacodyn. The. 126, 171.Google Scholar
  14. 14.
    Nustad, K. (1970) Br. J. Pharmac. 39, 87.Google Scholar
  15. 15.
    Nustad, K., and Rubin, J. (1970) Br. J. Pharmac. 40, 326.Google Scholar
  16. 16.
    Schachter, M., and Barton, S. (1974) Conference on Chemistry and biology of the kailikrein-kinin system in health and disease. Okt. 20-23.Google Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • K. Mann
    • 1
  • R. Geiger
    • 2
  • E. Werle
    • 2
  1. 1.I. Med. KlinikUniversität MunchenMunichGermany
  2. 2.Institut für Klinische Chemie und Klinische BiochemieUniversität MunchenMunichGermany

Personalised recommendations