A Sensitive Kinin Liberating Assay for Kininogenase in Rat Urine, Isolated Glomeruli and Tubules of Rat Kidney
There are several hints, that the kallikrein-kinin-system of the kidney is involved in physiological regulation mechanisms and in pathological processes (1, 2, 3, 4, 5, 6, 7). A prerequisite to evaluate the significance of the system, is the possibility of measuring kallikrein activities in tissues, where it is formed, excreted and inactivated. Recently Nustad and Pierce (8) showed, that the rat kidney synthesizes four kallikreins, which are released unchanged into urine. But the localisation of kallikreins in the nephron is not known. Moreover, there is no specific rat-urine kallikrein assay available, which is sensitive enough to measure enzyme activities in isolated structures of the kidney. This report will be focused on these points.
KeywordsDeoxycholic Acid Incubation Volume Urine Kallikrein International Congress Series Double Diffusion Test
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