The Regulation of Kallikrein Secretion from Isolated Submandibular Gland Slices by Neurotransmitters, Cyclic Nucleotides and Calcium
Evidence suggests that neurotransmitters interact with specific receptors on the plasma membrane of cells which results in the formation within the cell of a chemical messenger. Enzyme secretion in exocrine glands is considered to be controlled by the chemical messengers, adenosine 3′5′ monophosphate (cyclic AMP), guanosine 3′5′ cyclic monophosphate (cyclic GMP) and calcium. This second messenger concept was examined using isolated guinea-pig submandibular gland slices, incubated in modified Krebs-Ringer at 37°C and gassed with a mixture of 95% 02–5% CO2 (Fig. 1). The secretion of kallikrein into the incubation medium together with the morphological changes which accompany the secretory process were determined in response to noradrenaline, acetylcholine, dibutyryl cyclic AMP and dibutyryl cyclic GMP.
KeywordsAcinar Cell Secretory Granule Cyclic Nucleotide Enzyme Secretion Exocrine Gland
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