Methionyl-Lysyl-Bradykinin: The Kinin Released by Pepsin from Human Kininogens
Since Croxatto and coworkers (1, 2). first described and named the hypotensive, leiomyokinetic peptide released from mammalian serum by pepsin, the identity of pepsitocin has been in question. Habermann and coworkers (3, 4) reported the isolation of two related peptides from pepsin digests of highly purified bovine kininogen: MLBK-Ser-Val-Gln1 (90%) and MLBK-Ser-Val-Gln-Val-Met (10%). In contrast, Hochstrasser and Werle (5) found Gly (or Ser)-Arg-MLBK in pepsin digests of bovine plasma Fraction IV-6. Inasmuch as we had in hand appreciable amounts of highly purified human plasma kininogens (6), we undertook this study of the identity of pepsitocin.
KeywordsRetention Volume Pepsin Digest Porcine Pepsin Mammalian Serum Peptic Peptide
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