Adrenergic and Cholinergic Control of the Activation of the Kallikrein-Kinin System in the Rat Blood
reproducible in vitro in rat whole oxaleted blood (6);
not obtained in cell-free plasma nor in plasma containing either platelets, eosinophils, lymphocytes or erythrocytes (7);
effectiveness: epinephrine > nor-epinephrine; isoprenaline ineffective, does not block the action of epinephrine (6);
inhibited by either alpha or beta-adrenergic antagonists, the former being more potent (6);
inhibited by acetylsalicylic acid (Aspirin), bovine kallikrein inhibitor (Trasylol) or soy bean trypsin inhibitor (SBTI) (6,7).
reproducible in rat plasma treated with rat peritoneal fluid cell (PFC) suspensions exposed to epinephrine for 2 min.; not obtained with untreated PFC; after activation by epinephrine, PFC can be centrifuged and washed without loss of their acquired activity (8);
not obtained when PFC free of mast cells are added to plasma in the presence of epinephrine (8);
inhibited by dibutyril cyclic-AMP, 5 × 10−5M (9);
possibly associated with mast cell vacuolization and intracellular granule retraction, a phenomenon observed in epinephrine or nor-epinephrine-treated rat mesentery mast cells (10) which is inhibited by Aspirin (8), at the same concentrations which block kininogen depletion;
epinephrine activation of kallikrein in the presence of PFC is not accompanied by the release of histamine from such cells. Increased amounts of histamine are however observed in the tissues of epinephrine-treated rats (11, 12); they are to be attributed to enhanced, short-term synthesis of histamine induced by epinephrine, (Rosengren & Svensson) (13);
kininogen depletion and mast cell vacuolization apparently identical to those produced by catecholamines, are shown in rats treated with carbamylcholine, a cholinergic agonist. The effect on kininogen can also be shown in vitro, in oxalated blood incubated for 5 min. with carbamylcholine. This excludes reflexly released catecholamines as mediators of kallikrein activation in carbamylcholine treated rats.
KeywordsMast Cell Cholinergic Agonist Sympathomimetic Amine Kallikrein Activation Catecholamine Activation
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