Kinins pp 151-156 | Cite as

Purification of Kallikrein from Cat Submaxillary Gland

  • Chiaki Moriwaki
  • Yoshio Hojima
  • M. Schachter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 70)


Cat submaxillary gland has been used in many investigations on kallikrein-kinin system. Though it has not yet been established, the physiological functions of kallikreins in various glands, such as the functional vasodilatation, have been studied mainly in cat submaxillary gland (1, 2, 3). Furthermore, the secretory cells or subcellular distribution has been studied in this gland (4). Meanwhile the isolation of submaxillary kallikrein has not yet been achieved. In a series of investigation on the proteases in the rat submaxillary gland, Ekfors, et al. obtained a kallikrein-like peptidase (5), but the identity of the enzyme with kallikrein was not clear because of the lack of study on the biological activity of the preparation. Fiedler, et al. described that hog submaxillary kallikrein was separated into a number of active components with isoelectric points in the range pH 3.3–4.4 (6), but the specific activity of their preparation was less than a half of that of purified hog pancreatic kallikrein. We attempted the isolation of kallikrein from the cat submaxillary gland and obtained a highly purified preparation which was homogeneous in disc electrophoresis.


Submaxillary Gland Active Fraction Ammonium Formate Final Preparation Single Protein Band 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Chiaki Moriwaki
    • 1
  • Yoshio Hojima
    • 1
  • M. Schachter
    • 2
  1. 1.Faculty of Pharmaceutical SciencesScience University of TokyoTokyoJapan
  2. 2.Department of PhysiologyUniversity of AlbertaEdmontonCanada

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