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The Usefulness of Studies In Vitro for Understanding Cerebral Metabolite Transport In Vivo

  • Abel Lajtha
  • Miriam Banay-Schwartz
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Arguments between proponents of experimentation in vivo and those who favor studying systems in vitro are quite old. The former say that complex living systems cannot be studied in vitro because the changes in these preparations cause observations of artifacts, while the latter say that complex systems have first to be broken into simpler ones before we can hope for meaningful interpretations of our observations. We belong to what we perceive to be the majority, who feel that both approaches are very useful and necessary for studies of nervous system function, and are aware of the need to know the advantages and limitations of both. Similar discussions can still be heard among researchers studying neural barriers: “the blood-brain barrier (and other brain barriers) can be studied only in living animals; perhaps the biggest change in any system in vitro is the absence of the barrier)”, is the statement often heard. Clearly, if we want to study the initial rate of transport from the circulating plasma into brain, studies in vivo are the most suitable; but if we want to study the factors that determine the equilibrium and influence flux, cellular transport processes (requiring observations in vitro) should not be neglected. Hence this chapter.

Keywords

Olfactory Bulb Brain Research Brain Slice Amino Acid Transport Amino Acid Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Abel Lajtha
    • 1
  • Miriam Banay-Schwartz
    • 1
  1. 1.New York State Research Institute for Neurochemistry and Drug AddictionWard’s Island, New YorkUSA

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