Transport of Dopamine in Discrete Areas of the Striatum and of Cerebral Cortex in the Rat

  • J. P. Tassin
  • G. Blanc
  • L. Stinus
  • B. Berger
  • J. Glowinski
  • A. M. Thierry
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


For many years, the catecholaminergic innervation of the cerebral cortex in the rat was assumed to be only represented by the arborisations of the dorsal noradrenergic pathway. However, a quantity of dopamine (DA) slightly higher than that expected if DA was only the precursor of noradrenaline (NA) was found in this structure. It was thus postulated that the relatively high content of DA was related to the presence of dopaminergic terminals26. Two complementary approaches were available to test this hypothesis. The first one consisted of detecting some properties of dopaminergic terminals following the selective destruction of the noradrenergic ascending pathway. The second was to simultaneously identify specific properties of each type of catecholaminergic terminal in the cerebral cortex of normal rats. Since DA and NA are taken up in dopaminergic and noradrenergic terminals respectively by specific uptake processes, the estimation of the uptake of these amines could be used in these studies as an index of the specific properties of each type of catecholaminergic terminal.


Cerebral Cortex Brain Research Electrolytical Lesion Dopaminergic Innervation Dopaminergic Terminal 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Berger, B., Tassin, J.P., Blanc, G., Moyne, M.A., and Thierry, A.M., Histochemical confirmation for dopaminergic innervation of the rat cerebral cortex after destruction of the noradrenergic ascending pathways. Brain Research, 81 (1974) 332–337.CrossRefGoogle Scholar
  2. 2.
    Berger, B., Thierry, A.M., Tassin, J.P., and Moyne, M.A., Dopaminergic innervation of the rat prefrontal cortex: fluorescence histochemical study. Brain Research (1975) in press.Google Scholar
  3. 3.
    Bockaert, J., Fremont, J., Glowinski, J., Thierry, A.M., and Tassin, J.P., Topographical distribution of dopaminergic innervation and of dopaminergic receptors in the rat striatum: II. Distribution and characteristics of dopamine adenylate cyclase. Interaction of d-LSD with dopaminergic receptors. Brain Research, in press.Google Scholar
  4. 4.
    Breese, G.R, and Traylor, T.D., Effect of 6-hydroxydopamine on brain norepinephrine and dopamine: Evidence for selective degeneration of catecholamine neurons, J. Pharmacol, exp. Ther., 174 (1970) 413–420.Google Scholar
  5. 5.
    Coyle, J.T., and Snyder, S.H., Antiparkinsonian drugs: inhibition of dopamine uptake in the corpus striatum as a possible mechanism of action, Science, 166 (1969) 899–901.CrossRefGoogle Scholar
  6. 6.
    Coyle, J.T., and Axelrod, J., Development of the uptake and storage of 3H-NA in the rat brain, J. Neurochem., 18 (1971) 2061–2075.CrossRefGoogle Scholar
  7. 7.
    Fuxe, K., Hökfelt, T., Johansson, O., Jonsson, G., Lidbrink, P., and Ljungdahl, A. The origin of the dopaminergic nerve terminals in limbic and frontal cortex. Evidence for mesocortical dopamine neurons. Brain Research, 82 (1974) 349–355.CrossRefGoogle Scholar
  8. 8.
    Gauchy, C., Tassin, J.P., Glowinskl, J., and Cheramy, A., Isolation and radioenzymatic estimation of picogram quantities of dopamine and norepinephrine in biological samples, J. Neurochem., (1975) in press.Google Scholar
  9. 9.
    Glowinski, J., and Axelrod, J., Inhibition of uptake of 3H-norepi- nephrine in the intact rat brain by Imipramine and structurally related compounds. Nature (Lond), 204 (1964) 1318–1319.CrossRefGoogle Scholar
  10. 10.
    Glowinski, J., and Iversen, L.L., Regional studies of catecholamine in the rat brain. I. The disposition of 3H-NA, 3H-DA and 3H-DOPA in various regions of the brain, J. Neurochem., 13 (1966) 655–669.CrossRefGoogle Scholar
  11. 11.
    Hökfelt, T., Ljungdahl, A., Fuxe, K., and Johansson, O., Dopaminergic nerve terminals in the rat limbic cortex: aspects of the dopamine hypothesis of schizophrenia. Science, 184 (1974) 177–179.CrossRefGoogle Scholar
  12. 12.
    Iversen, L.L., The uptake of biogenic amines. In J.H. Biel and L.B. Abood (Eds.) Biogenic amines and physiological membranes in drus therapy. Marcel Dekker, New York, 1971, pp. 264–266.Google Scholar
  13. 13.
    Iversen, L.L., and Uretsky, N.J., Biochemical effects of 6-hydroxydopamine on catecholamine-containing neurons in the rat central nervous system. In T. Malmfors and H. Thoenen (Eds.), 6-Hydroxydo- pamine and catecholamine neurons. North Holland, Amsterdam, 1971, pp. 171–186.Google Scholar
  14. 14.
    Javoy, F., Sotelo, C., Herbet, A., and Agid, Y., Specificity of dopaminergic neuronal degeneration induced by intracerebral injection of 6-hydroxydopamine in the nigrostriatal dopamine system, Brain Research (1975) in press.Google Scholar
  15. 15.
    Jonsson, G., Pycok, Gh., Fuxe, K., and Sachs, Gh., Changes in the development of central noradrenaline neurons following neonatal administration of 6-hydroxydopamine, J. Neurochem. 22 (1974) 419–426.CrossRefGoogle Scholar
  16. 16.
    König, J.F.R., and Klippel, R.A., The rat brain: A stereotaxic atlas of the forebrain and lower parts of the brain stem, Williams and Wilkins, Baltimore, Md., 1963.Google Scholar
  17. 17.
    Leonard, C.M., The prefrontal cortex of the rat. I. Cortical projection of the mediodorsal nucleus. II. Efferent connections.. Brain Research, 12 (1969) 321–343.CrossRefGoogle Scholar
  18. 18.
    Lindvall, O., Björklund, A., Moore, R.Y., and Stenevi, V., Mesencephalic dopamine neurons projecting to neocortex, Brain Research, 59 (1974) 332–337.Google Scholar
  19. 19.
    Palkovits, M., Isolated removal of hypothalamic or other brain nuclei of the rat, Brain Research, 59 (1973) 449–450.CrossRefGoogle Scholar
  20. 20.
    Routtenberg, A., and Sloan, M., Self-stimulation in the frontal cortex of Rattus norvegicus, Behav. Biol., 7 (1972) 567–572.CrossRefGoogle Scholar
  21. 21.
    Snyder, S.H., and Coyle, J.T., Regional differences in 3H-norepi- nephrine and 3H-dopamine uptake into rat brain homogenates, J. Pharmacol, exp. Ther. 165 (1969) 78–86.Google Scholar
  22. 22.
    Tassin, J.P., Blanc G., Stinus, L., Berger, B., Glowinski, J. and Thierry, A.M., Distribution of dopaminergic terminals in the cerebral cortex, in preparation.Google Scholar
  23. 23.
    Tassin, J.P., Cheramy, A., Blanc, G., Thierry, A.M., and Glowinski, J., Topographical distribution of dopaminergic innervation and of dopaminergic receptors in the rat striatum: I. Micro-estimation of 3H-dopamine uptake and dopamine content in micro discs. Brain Research, in press.Google Scholar
  24. 24.
    Tassin, J.P., Thierry, A.M., Blanc, G., and Glowinski, J., Evidence for a specific uptake of dopamine by dopaminergic terminals of the rat cerebral cortex, Naunyn-Sch. Arch. Pharmacol, 282 (1974) 239–244.CrossRefGoogle Scholar
  25. 25.
    Thierry, A.M,, Blanc, G., Sobel, A., Stinus, L. and Glowinski, J., Dopaminergic terminals in the rat cortex. Science, 182 (1973) 499–501.CrossRefGoogle Scholar
  26. 26.
    Thierry, A.M., Stinus, L., Blanc, G., and Glowinski, J., Some evidence for the existence of dopaminergic neurons in the rat cortex. Brain Research, 50 (1973) 230–234.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • J. P. Tassin
    • 1
  • G. Blanc
    • 1
  • L. Stinus
    • 1
  • B. Berger
    • 1
  • J. Glowinski
    • 1
  • A. M. Thierry
    • 1
  1. 1.Groupe NB — College de FranceParis 50France

Personalised recommendations