Advertisement

Characteristics of the Uptake and Release of Glutamic Acid in Synaptosomes from Rat Cerebral Cortex. Effects of Ouabain

  • G. Takagaki
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

It is often described that glutamic acid seems probably to be a major excitatory transmitter in the mammalian CNS. Glutamic acid is very rapidly taken up from the medium into mammalian cerebral slices and synaptosomes. And it is released from either of them on depolarization effected by electrical pulses and elevated medium potassium. These properties of glutamic acid have been studied extensively and discussed in relation to its possible physiological transmitter role in CNS, although the in vitro techniques certainly involve highly unphysiological conditions 4, 8, 9.

Keywords

Glutamic Acid Glutamate Transport Amino Acid Fraction High Affinity Uptake System Cerebral Slice 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Berl, S., Takagaki, G., Clarke, D.D., and Waeisch, H., Metabolic compartments in vivo. Ammonia and glutamic acid metalDolism in brain and liver. J.biol.Chem., 237 (1962) 2562–2569.Google Scholar
  2. 2.
    Hamlberger, A., Amino acid uptake in neuronal and glial cell fractions from rabbit cerebral cortex. Brain Research, 31 (1971) 169–178.CrossRefGoogle Scholar
  3. 3.
    Hopkin, J., and Neal, M.J., Effect of electrical stimulation and high potassiiam concentrations on the efflux of [l4C]glycine from slices of spinal cord. Br. J. Pharmacol., 42 (1911) 215–223.Google Scholar
  4. 4.
    Lajtha, A., Amino acid transport in the brain in vivo and in vitro. In G.E.W. Wolstenholme, and D.W. Fitzsimons (Eds.), Aromatic Amino Acids in the Brain, Elsevier, Amsterdam, 1974, pp. 25–49.Google Scholar
  5. 5.
    Levi, G., and Raiteri, M., Detectability of high and low affinity uptake systems for GABA and glutamate in rat brain slices and synaptosomes. Life Sci., 12 (1973) 81–88.CrossRefGoogle Scholar
  6. 6.
    Matsui, S., and Yamamoto, C., Release of radioactive glutamic acid from thin sections of guinea-pig olfactory cortex in vitro. J. Neurochem., 24 (1975) 2145–250.CrossRefGoogle Scholar
  7. 7.
    Nicklas, W.J., Puszkin, S., and Berl, S., Effect of vinblastine and colchicine on uptake and release of putative transmitters by synaptosomes and on brain actomyosin-like protein. J.Neurochem., 20 (1973) 109–121.CrossRefGoogle Scholar
  8. 8.
    Quastel, J.H., Amino acids and the brain. Biochem.Soc.Trans., 2 (1974) 765–780.Google Scholar
  9. 9.
    Snyder, S.H., Young, A.B., Bennett, J.P. and Mulder, A.H., Synaptic biochemistry of amino acids. Federation Proc., 32 (1973) 2039–20147.Google Scholar
  10. 10.
    Takagaki, G., Developmental changes in glycolysis in rat cerebral cortex. J. Neurochem., 23 (1971) 1479–1487.Google Scholar
  11. 11.
    Takagaki, G., Uptake and release of glutamic acid in vitro in the mammalian CNS. Proc.Vth International Meeting of the International Society for Neurochemistry, Barcelona, 1975 p. 178.Google Scholar
  12. 12.
    Whittaker, V.P., and Barker, L.A., The subcellular fractionation of brain tissue with special reference to the preparation of synaptosomes and their component organelles. In R. Fried (ed.), Methods of Neurochemistry, Vol. 2, Marcel Dekker, New York, 1972, pp. 1–52.Google Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • G. Takagaki
    • 1
  1. 1.Department of NeurochemistryTokyo Metropolitan Institute for NeurosciencesFuchu-shi, TokyoJapan

Personalised recommendations