Kinetical Analysis of the Uptake of Glucose Analogs by Rat Brain Cortex Slices from Normal and Ischemic Brain
The present chapter describes experiments which were carried out in order to investigate the glucose transport across glial and neuronal cell membranes and the possible effect of ischemia upon the membrane transport. In order to clearly separate transport from metabolism, the two nonmetabolizable glucose analogs α-methyl-glucoside and 3-0-methyl-glucose were used10. The membrane transport was investigated by studying the uptake into the cellular compartment of brain cortex slices.
KeywordsExtracellular Space Ischemic Brain Membrane Transport Neuronal Cell Membrane Unidirectional Flux
Unable to display preview. Download preview PDF.
- 2.Bradbury, M.W.B., and Brøndsted, H.E., Sodium-dependent transport of sugars and iodide from the cerebral ventricles of the rabbit, J. Physiol., 234 (1973) 127–143.Google Scholar
- 4.International Critical Tables, Vol. 5, McGraw-Hill Book Company, New York, 1929, p. 71.Google Scholar
- 5.Joanny, P., Corriol, J., and Hillman, H., Uptake of monosaccharides by guinea-pig cerebral-cortex slices, Biochem. J., 112 (1969) 367–371.Google Scholar
- 6.Katzman, R., and Pappius, H.M., Brain electrolytes and fluid metabolism, Williams and Wilkins Company, Baltimore (1973).Google Scholar
- 7.Kjeldsen, C.S., and Lund-Andersen, H., Uptake of 2-deoxy-glucose by rat brain cortex slices. Transport versus phosphorylation. In preparation.Google Scholar
- 9.Lund-Andersen, H., and Kjeldsen, C.S., Uptake of glucose analogues by rat brain cortex slices, I. A kinetical analysis based upon a model, J. Neurochem., submitted.Google Scholar
- 10.Lund-Andersen, H., Kjeldsen, C.S,, Hertz, L., and Brøndsted, H.E,, Uptake of glucose analogues by rat brain cortex slices, II, Na+-independent membrane transport, J, Neurochem, submitted.Google Scholar
- 11.Møller, M., Møllgård, K., Lund-Andersen, H., and Hertz, L., Concordance between morphological and biochemical estimates of fluid spaces in rat brain cortex slices, Exp, Brain Res,, 22 (1974) 299–314.Google Scholar