Skip to main content

mRNA Specific Initiation Factors in the Control of Protein Synthesis

  • Chapter
Control of Gene Expression

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 44))

  • 90 Accesses

Abstract

Before mRNA was discovered it was commonly accepted that ribosomes in cells are specialized for the synthesis of specific proteins. Following the demonstration that the information for the amino acid sequence is, in fact, in the mRNA, ribosomes began to be viewed as non-specific organelles, capable of translating indiscriminately all information brought to them by mRNA. If this concept is true, all the controls of gene expression which must come into action when a cell adapts to new environmental conditions should be exclusively at the level of mRNA synthesis (transcription and maturation). To determine if translation control is at all a possible mechanism for the control of gene expression, it is therefore important to establish whether or not ribosomes are capable of discriminating between mRNAs and of translating preferentially one or another of the mRNAs present in the cytoplasm. This question is of course most relevant for mammalian cells, in which we know that (1) stable mRNAs function for prolonged periods of time (1) and (2) that more mRNA is synthesized in the nucleus than is actually translated on the cytoplasmic polyribosomes (see Scherrer, this volume). Since, however, the mechanism of protein synthesis is known in more detail in E.coli, we have concentrated much of our efforts in studying mRNA discrimination by E.coli ribosomes. Our experiments over the past three years have clearly demonstrated the existence of ribosome-associated protein factors which make the ribosome specialized for specific mRNAs. Recently, we have isolated similar factors from mammalian cells; these results will be presented in the second part of this paper.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

eBook
USD 9.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. M. REVEL & H.H. HIATT (1964). Proc. Natl. Acad. Sci. USA, 51, 810.

    Article  PubMed  CAS  Google Scholar 

  2. M. REVEL, In: The mechanism of protein synthesis and its regulation, L. Bosh Ed., North-Holland Publ. Co., Amsterdam, London (1972) pp. 87–131.

    Google Scholar 

  3. M. REVEL, H. GREENSHPAN E M. HERZBERG (1970). Eur. J. Biochem. 16, 117.

    Article  PubMed  CAS  Google Scholar 

  4. M. REVEL, Y. GRONER, Y. POLLACK, H. BERISSI & M. HERZBERG, In: Functional units in protein biosynthesis. (FEBS SYMPOSIUM) Acad. Press, New York, 23, (1972a) 237.

    Google Scholar 

  5. J.A. STEITZ (1969). Nature (London), 224, 967.

    Article  Google Scholar 

  6. Y. GRONER & M. REVEL (1971). Eur. J. Biochem. 22, 144.

    Article  PubMed  CAS  Google Scholar 

  7. J.W.B. HERSHEY, E. REMOLD O’DONNELL, D. KOLAKOFSKY, K.F. DEWEY & R.E. THACH In: Methods in Enzymology, Moldave and Grossman Eds., Acad. Press, New York, 20, (1971) 235.

    Google Scholar 

  8. S. OCHOA (1968). Naturwissenschaften, 11, 505.

    Article  Google Scholar 

  9. A.R. SUBRAMANIAN & B.D. DAVIS (1971). Nature New Biol. 228, 1254.

    Google Scholar 

  10. H.F. LODISH (1970a). Nature, 226, 705.

    Article  PubMed  CAS  Google Scholar 

  11. H.F. LODISH (1970b). J. Mol. Biol. 50, 689.

    Article  PubMed  CAS  Google Scholar 

  12. Y. GRONER, Y. POLLACK, H. BERISSI & M. REVEL. (1972b). Nature New Biol. 239, 16.

    PubMed  CAS  Google Scholar 

  13. Y. GRONER, Y. POLLACK, H. BERISSI & M. REVEL (1972a). FEBS LETTERS, 21, 223.

    Article  PubMed  CAS  Google Scholar 

  14. H.F. LODISH (1969). Nature, 224, 867.

    Article  PubMed  CAS  Google Scholar 

  15. S. LEE-IIUANG & S. OCHOA (1971). Nature New Biol. 234, 236.

    Google Scholar 

  16. M. YOSHIDA & P.S. RUDLAND (1972). J. Mol. Biol. 68, 465.

    Article  PubMed  CAS  Google Scholar 

  17. H. BERISSI, Y. GRONER & M. REVEL. (1971). Nature New Biol. 234, 44.

    PubMed  CAS  Google Scholar 

  18. M. REVEL, Y. POLLACK, Y. GRONER, R. SCHEPS, H. INOUYE, H. BERISSI & H. ZELLER In: Ribosomes, structures, function and biogenesis, FEBS Symposium, Vol. 27 (1972b) 261.

    Google Scholar 

  19. R. KAMEN, M. KONDO, W. ROMER & C. WEISSMANN (1972). Eur. J. Biochem. 31, 44.

    Article  PubMed  CAS  Google Scholar 

  20. F.W. STUDIER (1972). Science, 176, 367.

    Article  PubMed  CAS  Google Scholar 

  21. P. HERRLICH, M. SCIIWEIGER & W. SAUERBIER (1971). Molec. Gen. Genetics, 112,152.

    Article  CAS  Google Scholar 

  22. R.C. CALLAHAN & P. LEDER (1972). Arch. Biochem. Biophys. 153, 802.

    Article  PubMed  CAS  Google Scholar 

  23. R. SCHEPS & M. REVEL (1972). Eur. J. Biochem. 29, 319.

    Article  PubMed  CAS  Google Scholar 

  24. D.A. SHAFRITZ, P.M. PRICHARD & W.F. ANDERSON In: Methods in Molecular Biology, J.A. Last and A.I. Laskin, Eds., Marcel Dekker Inc., New york, 2,(1972) 265.

    Google Scholar 

  25. D.T. WIGGLE & A.E. SMITH (1973). Eur. J. Biochem. In press.

    Google Scholar 

  26. U. NUDEL, B. LEBLEU & M. REVEL (1973). Proc. Natl. Acad.Sci. USA, in press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1974 Plenum Press, New York

About this chapter

Cite this chapter

Revel, M., Groner, Y., Pollack, Y., Cnaani, D., Zeller, H., Nudel, U. (1974). mRNA Specific Initiation Factors in the Control of Protein Synthesis. In: Kohn, A., Shatkay, A. (eds) Control of Gene Expression. Advances in Experimental Medicine and Biology, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3246-6_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-3246-6_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3248-0

  • Online ISBN: 978-1-4684-3246-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics