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In Vivo and in Vitro Initiation of Transcription

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Control of Gene Expression

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 44))

Abstract

We have been trying to identify the sites of initiation of transcription in bacteriophage λ and to characterize the elements that control the transcription. The mRNAs were prepared both in vivo, by pulse labeling the phage-infected or induced lysogenic cells, and in vitro by using the λ DNA template and Escherichia cell RNA polymerase together with 32P-labeled nucleoside triphosphates. The promoter-proximal mRNA sequences were determined. The following observations were based on refs. 1–5.

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References

  1. BLATTNER, F.R. & DAHLBERG, J.E. (1972). Nature [New Biol.] 237, 227.

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  2. BLATTNER, F.R., DAHLBERG, J.E., BOETTIGER, J.K., FIANDT, M. & SZYBALSKI, W. (1972). Nature [New Biol.] 237, 232.

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  3. LOZERON, H.A., FUNDERBURGH, M.L., DAHLBERG, J.E., STARK, B.P. & SZYBALSKI, W. (1972). Abst. Ann. Meet. Am. Soc. Microbiol. p. 237.

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  4. DAHLBERG, J.E., LOZERON, H.A. & SZYBALSKI, W. Bacteriophage Meeting Cold Spring Harbor, N.Y., p. 86.

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  5. SZYBALSKI, W. (1972). In: LEDOUX, L.G.H. ( Ed.), “Uptake of informative molecules by living cells.” Amsterdam, North Holland Publishing Co. p. 59.

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© 1974 Plenum Press, New York

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Szybalski, W. (1974). In Vivo and in Vitro Initiation of Transcription. In: Kohn, A., Shatkay, A. (eds) Control of Gene Expression. Advances in Experimental Medicine and Biology, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3246-6_3

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  • DOI: https://doi.org/10.1007/978-1-4684-3246-6_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3248-0

  • Online ISBN: 978-1-4684-3246-6

  • eBook Packages: Springer Book Archive

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