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In Vivo and in Vitro Initiation of Transcription

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Control of Gene Expression

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 44))


We have been trying to identify the sites of initiation of transcription in bacteriophage λ and to characterize the elements that control the transcription. The mRNAs were prepared both in vivo, by pulse labeling the phage-infected or induced lysogenic cells, and in vitro by using the λ DNA template and Escherichia cell RNA polymerase together with 32P-labeled nucleoside triphosphates. The promoter-proximal mRNA sequences were determined. The following observations were based on refs. 1–5.

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© 1974 Plenum Press, New York

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Szybalski, W. (1974). In Vivo and in Vitro Initiation of Transcription. In: Kohn, A., Shatkay, A. (eds) Control of Gene Expression. Advances in Experimental Medicine and Biology, vol 44. Springer, Boston, MA.

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  • Print ISBN: 978-1-4684-3248-0

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