In Vivo and in Vitro Initiation of Transcription
We have been trying to identify the sites of initiation of transcription in bacteriophage λ and to characterize the elements that control the transcription. The mRNAs were prepared both in vivo, by pulse labeling the phage-infected or induced lysogenic cells, and in vitro by using the λ DNA template and Escherichia cell RNA polymerase together with 32P-labeled nucleoside triphosphates. The promoter-proximal mRNA sequences were determined. The following observations were based on refs. 1–5.
KeywordsCold Spring Harbor Transcriptional Control Operator Region North Holland Publishing Initial Sequence
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