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In Vivo and in Vitro Initiation of Transcription

  • W. Szybalski
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 44)

Abstract

We have been trying to identify the sites of initiation of transcription in bacteriophage λ and to characterize the elements that control the transcription. The mRNAs were prepared both in vivo, by pulse labeling the phage-infected or induced lysogenic cells, and in vitro by using the λ DNA template and Escherichia cell RNA polymerase together with 32P-labeled nucleoside triphosphates. The promoter-proximal mRNA sequences were determined. The following observations were based on refs. 1–5.

Keywords

Cold Spring Harbor Transcriptional Control Operator Region North Holland Publishing Initial Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    BLATTNER, F.R. & DAHLBERG, J.E. (1972). Nature [New Biol.] 237, 227.Google Scholar
  2. 2.
    BLATTNER, F.R., DAHLBERG, J.E., BOETTIGER, J.K., FIANDT, M. & SZYBALSKI, W. (1972). Nature [New Biol.] 237, 232.Google Scholar
  3. 3.
    LOZERON, H.A., FUNDERBURGH, M.L., DAHLBERG, J.E., STARK, B.P. & SZYBALSKI, W. (1972). Abst. Ann. Meet. Am. Soc. Microbiol. p. 237.Google Scholar
  4. 4.
    DAHLBERG, J.E., LOZERON, H.A. & SZYBALSKI, W. Bacteriophage Meeting Cold Spring Harbor, N.Y., p. 86.Google Scholar
  5. 5.
    SZYBALSKI, W. (1972). In: LEDOUX, L.G.H. ( Ed.), “Uptake of informative molecules by living cells.” Amsterdam, North Holland Publishing Co. p. 59.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • W. Szybalski
    • 1
  1. 1.McArdle Laboratory for Cancer ResearchUniversity of WisconsinMadisonUSA

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