In Vivo and in Vitro Initiation of Transcription

  • W. Szybalski
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 44)


We have been trying to identify the sites of initiation of transcription in bacteriophage λ and to characterize the elements that control the transcription. The mRNAs were prepared both in vivo, by pulse labeling the phage-infected or induced lysogenic cells, and in vitro by using the λ DNA template and Escherichia cell RNA polymerase together with 32P-labeled nucleoside triphosphates. The promoter-proximal mRNA sequences were determined. The following observations were based on refs. 1–5.


Cold Spring Harbor Transcriptional Control Operator Region North Holland Publishing Initial Sequence 


  1. 1.
    BLATTNER, F.R. & DAHLBERG, J.E. (1972). Nature [New Biol.] 237, 227.Google Scholar
  2. 2.
    BLATTNER, F.R., DAHLBERG, J.E., BOETTIGER, J.K., FIANDT, M. & SZYBALSKI, W. (1972). Nature [New Biol.] 237, 232.Google Scholar
  3. 3.
    LOZERON, H.A., FUNDERBURGH, M.L., DAHLBERG, J.E., STARK, B.P. & SZYBALSKI, W. (1972). Abst. Ann. Meet. Am. Soc. Microbiol. p. 237.Google Scholar
  4. 4.
    DAHLBERG, J.E., LOZERON, H.A. & SZYBALSKI, W. Bacteriophage Meeting Cold Spring Harbor, N.Y., p. 86.Google Scholar
  5. 5.
    SZYBALSKI, W. (1972). In: LEDOUX, L.G.H. ( Ed.), “Uptake of informative molecules by living cells.” Amsterdam, North Holland Publishing Co. p. 59.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • W. Szybalski
    • 1
  1. 1.McArdle Laboratory for Cancer ResearchUniversity of WisconsinMadisonUSA

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