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Regulation of the Expression of the Reovirus Genome in Vivo and in Vitro

  • George Acs
  • Daniel H. Levin
  • Michael Schonberg
  • Judith Christman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 44)

Abstract

Animal viruses enter their host cells as nucleoprotein complexes. The role of the nucleic acid portion of these nucleoproteins has been understood for some time, but the importance of the proteins has been appreciated only recently with the discovery that many animal viruses contain DNA and RNA polymerizing activities. Recognition of an RNA transcriptase activity within vaccinia virus (1), for instance, resolved the theoretical problems surrounding the mechanism(s) whereby a double-stranded DNA virus initiates RNA transcription in the cytoplasm of a eukaryotic cell. The finding that RNA tumor viruses contain enzymes which catalyze the synthesis of DNA (2, 3) resolved, at least in part, one pathway by which those viruses may induce stable malignant transformation of animal cells. Similarly, the mechanism by which the double-stranded RNA containing reoviruses initiates genetic function in animal cells remained obscure until Borsa and Graham (4) and Shatkin and Sipe (5) demonstrated an RNA transcriptase activity within the virion.

Keywords

Complementary Strand Sucrose Density Gradient Buoyant Density Transcriptase Activity Bromphenol Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • George Acs
    • 1
    • 2
  • Daniel H. Levin
    • 1
    • 2
  • Michael Schonberg
    • 1
    • 2
  • Judith Christman
    • 1
    • 2
  1. 1.Institute for Muscle DiseaseNew YorkUSA
  2. 2.Caroline Astell and Samuel C. SilversteinRockefeller UniversityNew YorkUSA

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