Regulation of the Expression of the Reovirus Genome in Vivo and in Vitro
Animal viruses enter their host cells as nucleoprotein complexes. The role of the nucleic acid portion of these nucleoproteins has been understood for some time, but the importance of the proteins has been appreciated only recently with the discovery that many animal viruses contain DNA and RNA polymerizing activities. Recognition of an RNA transcriptase activity within vaccinia virus (1), for instance, resolved the theoretical problems surrounding the mechanism(s) whereby a double-stranded DNA virus initiates RNA transcription in the cytoplasm of a eukaryotic cell. The finding that RNA tumor viruses contain enzymes which catalyze the synthesis of DNA (2, 3) resolved, at least in part, one pathway by which those viruses may induce stable malignant transformation of animal cells. Similarly, the mechanism by which the double-stranded RNA containing reoviruses initiates genetic function in animal cells remained obscure until Borsa and Graham (4) and Shatkin and Sipe (5) demonstrated an RNA transcriptase activity within the virion.
KeywordsComplementary Strand Sucrose Density Gradient Buoyant Density Transcriptase Activity Bromphenol Blue
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