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The Interaction of Uterine Estrogen Receptors with DNA

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 36)

Abstract

The binding of estrogen to specific cytoplasmic and nuclear “receptor” proteins in the uterus is now well established. Temporal analyses of nuclear and cytoplasmic receptor forms following the administration of estradiol indicate that, after its formation in the cell, the cytoplasmic hormone-receptor complex is transferred to the nucleus where it is associated with chromatin material (1–4).

Keywords

Estrogen Receptor Ammonium Sulfate Sucrose Gradient Assay Tube Sucrose Gradient Centrifugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Jensen, E.V., Suzuki, T., Kawashima, T., Stumpf, W.E., Jungblut, P.W., and DeSombre, E.R., A Two-Step Mechanism for the Interaction of Estradiol with Rat Uterus., Proc. Natl. Acad. Sci. U.S., 59: 632, 1968.CrossRefGoogle Scholar
  2. 2.
    Jensen, E.V., Suzuki, T., Numata, M., Smith, S., and DeSombre, E.R., Estrogen-binding Substances of Target Tissues, Steroids, 13: 417–427, 1969.PubMedCrossRefGoogle Scholar
  3. 3.
    Shyamala, G., and Gorski, J., Estrogen Receptors in the Rat Uterus, Studies on the Interaction of Cytosol and Nuclear Binding Sites, J. Biol. Chem.,244:1097–1103, 1969.Google Scholar
  4. 4.
    Giannopoulos, G., and Gorski, J., Estrogen Receptors, Quantitative Studies on Transfer of Estradiol from Cytoplasmic to Nuclear Binding Sites, J. Biol. Chem.,246:2524–2529, 1971.PubMedGoogle Scholar
  5. 5.
    Toft, D., The Association of the Uterine Estrogen Receptor with DNA., Endocrinology, 88(suppl.), A-215, 1971.Google Scholar
  6. 6.
    Toft, D., The Interaction of Uterine Estrogen Receptors with DNA, J. Steroid Biochem., 3:515–522, 1972.CrossRefGoogle Scholar
  7. 7.
    King, R.J.B., Beard, V., Gordon, J., Pooley, A.S., Smith, J.H., Steggles, A.W., and Vertes, M., Studies on Estradiol Binding in Mammalian Tissues, In Raspé, G. (ed.): Schering Workshop on Steroid Hormone Receptors, Advances in the Biosciences 7, Pergamon Press-Vieweg, pp. 21–37, 1971.Google Scholar
  8. 8.
    Musliner, T.A., and Chader, G.J., Estradiol Receptors of the Rat Uterus: Interaction of the Cytoplasmic Estrogen-Receptor with DNAin vitro, Biochim. Biophys. Acta,262: 256-l63, 1972.Google Scholar
  9. 9.
    Clemens, L.E., and Kleinsmith, L.J., Specific Binding of the Oestradiol-Receptor Complex to DNA., Nature,237: 201+-206, 1972.Google Scholar
  10. 10.
    Yamamoto, K.R., Multiple Forms of Estrogen Receptors and Their Interaction with DNA, 56th Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, 1972, Abstr. 1193.Google Scholar
  11. 11.
    Rochefort, H., Capony, F., and Inserm, J.A., Interaction of the Estradiol Cytosol Receptor of Uterus to Nuclear Components, IV. International Congress of Endocrinology, Washington, 1972, Abstr. 636.Google Scholar
  12. 12.
    Litman, R.M., A Deoxyribonucleic Acid Polymerase from Micrococcus Luteus Isolated on Deoxyribonucleic Acid-Cellulose, J. Biol. Chem.,243: 6222–6233, 1968.PubMedGoogle Scholar
  13. 13.
    Burton, K., A Study of the Conditions and Mechanism of the Diphenylamine Reaction for the Colorimetric Estimation of Deoxyribonucleic Acid, Biochem. J., 62: 315–323, 1956.Google Scholar
  14. 14.
    Marmur, J., A Procedure for the Isolation of Deoxyribonucleic Acid from Micro-organisms., J. Mol. Biol., 3: 208–218, 1961.CrossRefGoogle Scholar
  15. 15.
    Toft, D. (manuscript in preparation).Google Scholar
  16. 16.
    DeSombre, E.R., Chabaud, J.P., Puca, G.A., and Jensen, E.V., Purification and Properties of Estrogen-Binding Proteins from Calf Uterus, J. Steroid Biochem.,2:95–103, 1971.CrossRefGoogle Scholar
  17. 17.
    Musliner, T.A., Chader, G.J., and Villee, C.A., Studies on Estradiol Receptors of the Rat Uterus., Nuclear Uptakein vitro, Biochemistry, 9: 4448–4453, 1970.Google Scholar
  18. 18.
    Steggles, A.W., Spelsberg, T.C., Glasser, S.R., and O’Malley, B.W., Soluble Complexes Between Steroid Hormones and TargetTissue Receptors Bind Specifically to Target-Tissue Chromatin, Proc. Natl. Acad. Sci. U.S., 68: 1479–1482, 1971.CrossRefGoogle Scholar
  19. 19.
    O’Malley, B.W., Toft, D.O., and Sherman, M.R., Progesteronebinding Components of Chick Oviduct II., Nuclear Components, J. Biol. Chem.,246: 1117–1122, 1971.Google Scholar
  20. 20.
    Steggles, A.W., Spelsberg, T.C., and O’Malley, B. W., Tissue Specific Binding invitroof Progesterone-Receptor to the Chromatins of Chick Tissues, Biochem. Biophys. Res. Commun.,k3:20–27, 1971.CrossRefGoogle Scholar
  21. 21.
    Fang, S., and Liao, S., Androgen Receptors, Steroid and Tissue-Specific Retention of a 17ß-hydroxy-5ü-androstan-3-one Protein Complex by the Cell Nuclei of Ventral Prostate., J. Biol. Chem.,246: 16–24, 1971.PubMedGoogle Scholar
  22. 22.
    Spelsberg, T.C., Steggles, A.W., and O’Malley, B.W., Progesterone-binding Components of Chick Oviduct, III., Chromatin Acceptor Sites, J. Biol. Chem.,2464188–4197, 1971.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • D. Toft
    • 1
  1. 1.Department of Obstetrics and GynecologyVanderbilt University School of MedicineNashvilleUSA

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