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Interactions of LH and hCG with Testicular Gonadotropin Receptors

  • K. J. Catt
  • M. L. Dufau
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 36)

Abstract

Gonadal receptor sites for LH and hCG were originally demonstrated by morphological studies which employed a variety of labeled hormone preparations to show trophic hormone localization in cells concerned with steroid synthesis and secretion (1–4). Uptake of LH by the Leydig cells of the rat testis has been demonstrated in vivo (3), and in vitro with dispersions and cultures of the interstitial cell fraction of the testis (5,6). Interstitial cell dispersions prepared by teasing apart the testis tubules (7) retain the capacity for trophic hormone binding (6) and testosterone biosynthesis from labeled precursors (7,8), but show diminished quantitative responses to LH and hCG in terms of cyclic AMP (9) and testosterone production (10). The lability of the interstitial cells to physical dispersion is reflected in the corresponding loss of hormone-sensitive adenylate cyclase activity during homogenization of the adult rat testis (10), and may reflect the presence of an inhibitory factor released during physical separation of the testis. In contrast, Leydig cells prepared by collagenase digestion of the rat testis display relatively high sensitivity to hCG stimulation in vitro.

Keywords

Leydig Cell Testosterone Production Trophic Hormone Testis Receptor Intact Testis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • K. J. Catt
    • 1
  • M. L. Dufau
    • 1
  1. 1.Reproduction Research BranchNational Institutes of Child Health and Human Development, National Institutes of HealthBethesdaUSA

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