Assay of Platelet ATP and ADP by the Luciferase Method: Some Theoretical and Practical Aspects
The metabolism and release of platelet adenine nucleotides apparently have a critical influence on platelet aggregation in vitro and probably on the formation of hemostatic plug and platelet thrombus in vivo. It is thus of the greatest importance to be able to determine platelet ATP and ADP, whether or not released during aggregation, by means of a highly reproducible and sensitive assay. The firefly method enables ATP and ADP to be assayed in the same small volumes of PRP or platelet suspension as used in screening tests, e.g. aggregation tested with the usual agents (1–7). These volumes range from 1.0 to 2.0 ml and contain approximately 3 × 105 platelets/mm3.
KeywordsEthanol Extract Light Emission Pyruvate Kinase Usual Agent Platelet Suspension
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