Measurement of Platelet Aggregation and Thrombus Formation in Circulating Blood

  • G. Hornstra
  • S. Y. Gielen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)


Platelet thrombi are of great significance both as a complicating (1) and as an initiating (2) factor in atherosclerosis. This relation mainly defines the demands to he made on a platelet aggregation test when used in atherosclerosis research. Such a test should render it possible to measure platelet aggregation in the arterial circulation and, moreover, should enable the investigation of the time dependency of an effect on the aggregation process within each animal, without blood loss. The “filter-loop technique”, described below, meets these demands completely.


Platelet Aggregation Thrombus Formation Aggregation Index Platelet Thrombus Aggregation Measurement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Harland W. A., The pathogenesis of myocardial infarction and coronary-thrombosis. In: S. Sherry, K. M. Brinkhous, E. Gento and J. M. Stengle (Eds): Thrombosis. National Academy of Sciences, Washington D. C., 1969, p. 126.Google Scholar
  2. 2.
    Heggtveit H. A., Atheromatous transformation of intracardiac mural thrombi. Amer. J. Pathol. 52, 70a, 1968.Google Scholar
  3. 3.
    Hornstra G., Method to determine the degree of ADP-induced platelet ag-gregation in circulating rat blood (filter-loop technique). Brit. J. Haematol. 19, 321, 1970.CrossRefGoogle Scholar
  4. 4.
    Hornstra G., Degree and duration of prostaglandin E1-induced inhibition of platelet aggregation in the rat. European J. Pharmacol. 15, 343, 1971.Google Scholar
  5. 5.
    Hornstra G., Starrenburg A., Induction of experimental arterial occlusive thrombin in rats: technique, histology and utility (to be published).Google Scholar
  6. 6.
    Hornstra G., The influence of sunflowerseed oil and hardened coconut oil on intra-arterial occlusive thrombosis in rats. Nutr. Metabol. 13, 140, 1971.CrossRefGoogle Scholar
  7. 7.
    O’brien J. R., Platelet aggregation I. Some effects of the adenosine phosphates, thrombin and cocain upon platelet adhesiveness. J. Clin. Pathol. 15, 446, 1962.PubMedCrossRefGoogle Scholar
  8. 8.
    Rozenberg M. C., Holmsen H., Adenine nucleotide metabolism of blood platelets. IV. Plate, let-aggregation response to exogenous ATP and ADP. Biochim. Biophys. Acta 157, 280, 1968.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • G. Hornstra
    • 1
  • S. Y. Gielen
    • 1
  1. 1.Unilever ResearchVlaardingenThe Netherlands

Personalised recommendations