Measurement of Platelet Aggregation and Shape Change

  • F. Michal
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)


Platelet aggregation and shape change are possibly the only platelet functions which can be measured in vitro with a degree of reliability and validity. The nephelometric method of Born (3) has proven to be one of the most valuable tools in platelet research. The method takes advantage of the changes in the optical density of platelet—rich plasma (PRP), or platelets suspended in an appropriate “plasma-free” medium, when a substance causing them to clump is added. Changes in light transmission which, within limits, are directly proprotional to the concentration of the aggregating agent, can be recorded photometrically. When the temperature of the system and the collision forces between platelets are controlled (6), it is possible to measure platelet aggregation in a highly quantitative manner (4, 9).


Platelet Aggregation Light Transmission Adenosine Diphosphate Trisodium Citrate Incident Light Beam 
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Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • F. Michal
    • 1
  1. 1.M.R.C. Thrombosis Research Group, Dept. of PharmacologyRoyal College of SurgeonsLondonUK

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