Hemoglobin Function During Blood Storage XV: Effects of Metabolic Additives Inosine and Methylene Blue on p50 and 2,3-DPG
The major purpose of transfusing whole blood or red cells is to provide the recipient with an improved ability to transport oxygen to the tissues. Hemoglobin has this capability, but in most mammalian red cells-including the human-2,3-diphosphoglycerate (2,3-DPG) is required for normal oxygen release to the tissues (Chanutin, 1966; Chanutin and Curnish, 1967; Benesch & Benesch, 1967). After the discovery of the mediator of-or cofactor for-normal hemoglobin function; that is, 2,3-DPG, the first studies which looked at this compound in the red cell and the oxygen affinity of hemoglobin during liquid blood storage showed that 2,3-DPG decreased very rapidly during storage in acid citrate dextrose (ACD). The oxygen affinity of hemoglobin increased at the same rate as shown by the decrease in p50 (an inverse though direct measure of oxygen affinity) which was similar in slope of decrease to the decrease in 2,3-DPG during the several weeks which were studied (Akerblom, et al, 1968; Bunn, H. F., et al, 1969). The early work of Chanutin and his coworkers which goes back to 1958, and other studies which relate to the importance of 2,3-DPG for the normal position of the oxyhemoglobin dissociation curve have been reviewed more thoroughly in the preceedings from the first International Symposium on Red Cell Metabolism and Function (Dawson, 1970).
KeywordsMethylene Blue Storage Period Pentose Phosphate Pathway Oxygen Affinity Liquid Storage
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