Rejuvenation and Freezing of Outdated Human Red Cells

  • C. Robert Valeri
  • Charles G. Zaroulis
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)


The 24-hour posttransfusion survival value, the mechanism of removal of the nonviable compatible red cells, the spontaneous hemolysis that occurs in vitro with storage, and the potential toxicity of additives used in the preservation procedure have all served as criteria for satisfactory preservation of human red cells (Valeri, 1970a and 1971). Of current concern is the ability of preserved red cells to maintain normal oxygen transport function at the time of transfusion.


Uric Acid Level Disodium Phosphate Mmoles Glucose Oxyhemoglobin Dissociation Curve Oxygen Transport Function 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Åkerblom, O. (1967). ONR Report DR-143:144.Google Scholar
  2. Åkerblom, O., deVerdier, C. H., Garby, L., and Hogman, C. (1968). Scand. J. Clin. Lab. Invest. 21: 245.PubMedCrossRefGoogle Scholar
  3. Benesch, R., and Benesch, R. E. (1967). Biochem. Biophys. Res. Commun. 26: 162.PubMedCrossRefGoogle Scholar
  4. Benesch, R., and Benesch, R. E. (1969). Nature (London) 221: 618.CrossRefGoogle Scholar
  5. Bellingham, A. J., and Huehns, E. R. (1969). Forsvarsmedicin 5: 207.Google Scholar
  6. Beutler, E., and Wood, L. (1969). J. Lab. Clin. Med. 74: 300.PubMedGoogle Scholar
  7. Bunn, H. F., May, M. H., Kocholaty, W. F., and Shields, C. E. (1969). J. Clin. Invest. 48: 311.PubMedCrossRefGoogle Scholar
  8. Chanutin, A., and Curnish, R. R. (1967). Arch. Biochem. 121: 96.PubMedCrossRefGoogle Scholar
  9. Chanutin, A. (1967). Transfusion 7: 120.PubMedCrossRefGoogle Scholar
  10. Dawson, R. B., Jr., Kocholaty, W. F., and Gray, J. L. (1970). Transfusion 10: 299.PubMedCrossRefGoogle Scholar
  11. Dawson, R. B., Jr., and Ellis, T. J. (1970). Transfusion 10: 113.PubMedCrossRefGoogle Scholar
  12. Dawson, R. B., Jr., and Kocholaty, W. F. (1971). Am. J. Clin. Path. 56: 656.PubMedGoogle Scholar
  13. Deuticke, B., Duhm, J., and Dierkesmann, R. (1971). Pflugers. Arch. 326: 15.PubMedCrossRefGoogle Scholar
  14. Duhm, J., Deuticke, B., and Gerlach, E. (1971). Transfusion 11: 147.PubMedCrossRefGoogle Scholar
  15. Fortier, N. L., Hirsch, N. M., and Valeri, C. R. (1969). Forsvarsmedicin 5: 250.Google Scholar
  16. Gibson, J. G., II, Murphy, W. P., Jr., Seheitlin, W. A., and Bees, S. B. (1956). Amer. J. Clin. Path. 26: 855.Google Scholar
  17. Gibson, J. G., 2nd, Rees, S. B., McManus, T. J., and Seheitlin, W. A. (1957). Amer. J. Clin. Path. 28: 569.Google Scholar
  18. Hilpert, P., Fleischmann, R. G., Kempe, D., and Bartels, H. (1963). Am. J. Physiol. 205: 337.PubMedGoogle Scholar
  19. Högman, C. F., and Åkerblom, O. (1969). Forsvarsmedicin 5: 247.Google Scholar
  20. Kalchar, H. M. (1947). J. Biol. Chem. 167: 429.Google Scholar
  21. Kopriva, C. J., Ratliff, J. L., Fletcher, J. R., Fortier, N. L., and Valeri, C. R. (in press). Ann. Surg.Google Scholar
  22. McCoon, R., and Derrick, J. B. (1972). Anesthesiology 36: 119.CrossRefGoogle Scholar
  23. McManus, T. J., and Borgese, T. A. (1961). Fed. Proc. 20: 65.Google Scholar
  24. Meryman, H. T., and Hornblower, M. (in press). TransfusionGoogle Scholar
  25. Oski, F. A., Travis, S. F., Miller, L. D., Delivoria-Papadopoulos, M., and Cannon, E. (1971). Blood 37: 52.PubMedGoogle Scholar
  26. Paniker, N. V., and Beutler, E. (1971). J. Lab. Clin. Med. 78: 472.PubMedGoogle Scholar
  27. Proctor, H. J., Parker, J. C., and Johnson, G., Jr. (1971). Ann. Surg. 173: 357.PubMedCrossRefGoogle Scholar
  28. Rapoport, S. (1947). J. Clin. Invest. 26: 591.CrossRefGoogle Scholar
  29. Runck, A. H., and Valeri, C. R. (in press). Transfusion.Google Scholar
  30. Seidl, S., and Spielmann, W. (1970). Modern Problems of Blood Preservation, Edited by W. Spielmann and S. Seidl. Stuttgart, G. Fischer, pp 125–137.Google Scholar
  31. Shafer, A. W., Tague, L. L., Welch, M. H., and Guenter, C. A. (1971). J. Lab. Clin. Med. 77: 430.PubMedGoogle Scholar
  32. Shields, C. E., Lopas, H., and Birndorf, N. I. (1970). J. Clin. Pharmacol. 10: 316.CrossRefGoogle Scholar
  33. Shields, C. E. (1971). Transfusion 11: 134.PubMedCrossRefGoogle Scholar
  34. Siegel, D. C., Reed, P. C., Fresquez, V., Cochin, A., and Moss, G. S. (1971). Ann. Surg. 174: 932.PubMedCrossRefGoogle Scholar
  35. Simon, E. R., Chapman, R. G., and Finch, C. A. (1962). J. Clin. Invest. 141: 351.CrossRefGoogle Scholar
  36. Strumia, M. M., Strumia, P. V., and Eusebi, A. J. (1970). J. Lab. Clin. Med. 76: 907.PubMedGoogle Scholar
  37. Strumia, M. M., and Strumia, P. V. (1971). Transfusion 11: 258.PubMedCrossRefGoogle Scholar
  38. Valeri, C. R., and Hirsch, N. M. (1969). J. Lab. Clin. Med. 73: 722.PubMedGoogle Scholar
  39. Valeri, C. R. (1970a). Crit. Rev. Clin. Lab. Sci. 1: 381.CrossRefGoogle Scholar
  40. Valeri, C. R. (1970b). Modern Problems of Blood Preservation, Edited by W. Spielmann and S. Seidl. Stuttgart, G. Fischer, pp 125–137.Google Scholar
  41. Valeri, C. R., Szymanski, I. O., and Runck, A. H. (1970). Transfusion 10: 102.PubMedCrossRefGoogle Scholar
  42. Valeri, C. R. (1971). New Engl. J. Med. 284: 81.PubMedCrossRefGoogle Scholar
  43. Valeri, C. R., and Collins, F. B. (1971). J. Appl. Physiol. 31: 823.PubMedGoogle Scholar
  44. Valeri, C. R., and Kopriva, C. J. (in press). Oklahoma Shock Symposium Proceedings, Plenum Press, New York.Google Scholar
  45. Valeri, C. R., Szymanski, I. O., and Zaroulis, C. G. (in press). Vox. Sang.Google Scholar
  46. Valtis, D. J., and Kennedy, A. C. (1953). Glasgow Med. J. 34: 521.PubMedGoogle Scholar
  47. Valtis, D. J., and Kennedy, A. C. (1954). Lancet 1: 119.CrossRefGoogle Scholar
  48. Wood, L., and Beutler, E. (1971). Transfusion 11: 123.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • C. Robert Valeri
    • 1
  • Charles G. Zaroulis
    • 1
  1. 1.Naval Blood Research LaboratoryChelseaUSA

Personalised recommendations