Advertisement

The Two Bohr Effects: Physiological Consequences of Ligand Interaction with Hemoglobin

  • B. Wranne
  • R. Woodson
  • J. Detter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)

Abstract

Factors affecting oxygen transport to tissue are cardiac output and its distribution, blood oxygen content, and affinity of hemoglobin for oxygen. Hemoglobin-oxygen affinity, in turn, depends upon pH, CO2, and DPG. The physiologic significance of such alterations in oxygen affinity of hemoglobin has been a matter of some interest. In the course of studying the in vivo consequences of increased hemoglobin-oxygen affinity, we recently noted that rats with a 15 mm Hg decrease in PO2 due to DPG depletion were able to perform in a maximal exercise test as well or very nearly as well as rats with normal hemoglobin-oxygen affinity. By contrast, rats with normovolemic anemia showed a marked reduction in work capacity.

Keywords

Base Excess Oxygen Affinity Maximal Exercise Test Human Haemoglobin Bohr Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bauer, C. Antagonistic influence of CO2 and 2,3 diphosphoglycerate on the Bohr effect of human haemoglobin. Life Sci. 8: 1041–1046, 1969.PubMedCrossRefGoogle Scholar
  2. 2.
    Bauer, C. Reduction of the carbon dioxide affinity of human haemoglobin solutions by 2,3 diphosphoglycerate. Resp. Physiol. 10: 10–19, 1970.CrossRefGoogle Scholar
  3. 3.
    Garby, L., M. Robert, and B. Zaar. Proton and carbamino– linked oxygen affinity of normal human blood. Acta Physiol. Scand. 1972 (In Press).Google Scholar
  4. 4.
    Lenfant, C., P. Ways, C. Aucutt and J. Cruz. Effect of chronic hypoxic hypoxia on the dissociation curve and respiratory gas transport in man. Resp. Physiol. 7: 7–29, 1969.CrossRefGoogle Scholar
  5. 5.
    Naeraa, N., E.S. Petersen, E. Boye and J.W. Severinghaus. pH and molecular CO2 components of the Bohr effect in human blood. Scand. J. Clin. Lab. Invest. 18: 96–102, 1966.PubMedCrossRefGoogle Scholar
  6. 6.
    Parker, J.C. Influence of 2,3-diphosphoglycerate metabolism on sodium-potassium permeability in human red blood cells: studies with bisulfite and other redox agents. J. Clin. Invest. 48: 117–125, 1969.PubMedCrossRefGoogle Scholar
  7. 7.
    Siggaard-Andersen, O. Blood acid-base alignment nomogram. Scales for pH, Pcobase excess of whole blood of different hemoglobin concentrations, plasma bicarbonate, and plasma total-CO2. Scand. J. Clin. Lab. Invest. 15: 211–217, 1963.CrossRefGoogle Scholar
  8. 8.
    Siggaard-Andersen, O. Oxygen-linked hydrogen ion binding of human hemoglobin. Effects of carbon dioxide and 2,3-diphosphoglycerate. I. Studies on erythrolysate. Scand. J. Clin. Lab. Invest. 27: 351–360, 1971.PubMedCrossRefGoogle Scholar
  9. 9.
    Siggaard-Andersen, O. and N. Sailing. Oxygen-linked hydrogen ion binding of human hemoglobin. Effects of carbon dioxide and 2,3-diphosphoglycerate. II. Studies on whole blood. Scand. J. Clin. Lab. Invest. 27: 361–366, 1971.PubMedCrossRefGoogle Scholar
  10. 10.
    Tomita, S. and A. Riggs. Studies of the interaction of 2,3- diphosphoglycerate and carbon dioxide with hemoglobins from mouse, man, and elephant. J. Biol. Chem. 246: 547–554, 1971.PubMedGoogle Scholar
  11. 11.
    Torrance, J., P. Jacobs, A. Restrepo, J. Eschbach, C. Lenfant and C.A. Finch. Intraerythrocytic adaptation to anemia. N. Engl. J. Med. 283: 165–169, 1970.PubMedCrossRefGoogle Scholar
  12. 12.
    Wranne, B., R.D. Woodson arid J.C. Detter. The Bohr effect: interaction between H+, CO2, and 2,3-DPG in fresh and stored blood. J. Appl. Physiol. 1972 (In Press).Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • B. Wranne
    • 1
    • 2
  • R. Woodson
    • 1
    • 2
  • J. Detter
    • 1
    • 2
  1. 1.Department of MedicineUniversity of Washington School of MedicineUSA
  2. 2.Department of Laboratory MedicineUniversity of Washington School of MedicineUSA

Personalised recommendations