Oxygen Affinity Independent Action of Cyanate and 2,3 DPG on Sickling

  • Michael C. Jensen
  • H. Franklin Bunn
  • George C. Halikas
  • David G. Nathan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)

Abstract

Since the Hb S oxygen dissociation curve is shifted to lower partial pressures by carbamylation, it seems important to know whether the cyanate induced in vitro inhibition of sickling is completely or partially due to this increase in oxygen affinity. In order to detect a possible direct interference of carbamyl groups with the aggregation of deoxy-hemoglobin S molecules, sickling was compared in carbamylated and noncarbamylated sickle cells, and the number of sickled forms and the actual oxygen saturations of the hemoglobin were determined simultaneously. These studies have shown that the cyanate effect on sickling is partially independent of the effect of cyanate on oxygen affinity.

Keywords

Formaldehyde Albumin Anemia Pyruvate Acetyl 

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References

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    May, A., A.J. Bellingham, E.R. Huehns, and G.H. Beaven: Lancet 1: 658, 1972.PubMedCrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • Michael C. Jensen
    • 1
  • H. Franklin Bunn
    • 1
  • George C. Halikas
    • 1
  • David G. Nathan
    • 1
  1. 1.Children’s Hospital Medical CenterHarvard Medical SchoolUSA

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