Effect of Inorganic Phosphate on Red Cell Metabolism: In Vitro and In Vivo Studies

  • Michael C. Brain
  • Robert T. Card
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)


The oxygen dissociation curve of whole blood is influenced by pH and the levels of 2,3-DPG and ATP in the red cells. The levels of these compounds are closely related to red cell metabolism which is determined by a number of factors including pH, temperature, Pi concentration, pO2 and pCO2. A left shift in the hb-O2 dissociation curve has been correlated to low levels of ATP and 2,3-DPG in hypophosphatemic states (Lichtman et al, 1971), conversely, a right shift in the curve has been observed with high levels of ATP and 2,3-DPG in association with the hyperphosphatemia of renal disease (Lichtman and Miller, 1970). These observations suggest that the metabolism of the red cell is influenced by relatively minor variations in Pi concentration.


Plasma Phosphate Oxygen Dissociation Curve Adenine Phosphoribosyltransferase Vivo Study Fresh Human Blood 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bergmeyer, H.-U. (1965). “Methods of Enzymatic Analysis”, Academic Press, New York and London, pp 266.Google Scholar
  2. Brewer, G.J., Eaton, J.W., Weil, J.V. and Grover, R.F. (1970), in Brewer, G.J., ed. “Red Cell Metabolism and Function”, Plenum Press, New York, pp 95.Google Scholar
  3. Chen, P.S., Toribara, T.Y. and Warner, H. (1956). Anal. Chem. 28s 1756.CrossRefGoogle Scholar
  4. Eckel, R.E., Rizzo, S.C., Lodish, H. and Berggren, A.B. (1966). Am. J. Physiol. 210: 737.PubMedGoogle Scholar
  5. Greenberg, B.G., Winters, R.W. and Graham, J.B. (1960). J. Clin. End. Metab. 20: 364.CrossRefGoogle Scholar
  6. Guest G.M. and Brown, E.W. (1957). Am. J. Dis. Child. 93: 486.Google Scholar
  7. Keitt, A.S. (1971a). Personal communication.Google Scholar
  8. Keitt, A.S. (1971b). J. Lab. Clin. Med. 77: 470.PubMedGoogle Scholar
  9. Lichtman, M.A. and Miller, D.R. (1970). J. Lab. Clin. Med. 76: 267.PubMedGoogle Scholar
  10. Lichtman, M.A., Miller, D.R., Cohen, J. and Waterhouse, C. (1971). Ann. Int. Med. 74: 562.PubMedGoogle Scholar
  11. Lowry, O.H., Passoneau, J.V., Hasselberger, F.X. and Schulz, D.W. (1964). J. Biol. Chem. 239: 18.PubMedGoogle Scholar
  12. Minakami, S. and Yoshikawa, H. (1966). J. Biochem. ( Tokyo ), 59: 145PubMedGoogle Scholar
  13. Rose, I. and Warms, J.V.B. (1966). J. Biol. Chem. 241: 4848.PubMedGoogle Scholar
  14. Torrance, J., Jacobs, P., Restrepol A., Eschbach, J., Lenfant, C. and Finch, C.A. (1970). N. Engl. J. Med. 283: 165.PubMedCrossRefGoogle Scholar
  15. Vestergaard-Bogind, B. (1962). Scand. J. Clin, and Lab, Invest. 14: 461.CrossRefGoogle Scholar
  16. Vestergaard-Bogind, B. and Scharff, O. (1968), in Deutsch, E., Gerlach, E. and Moser, K., eds. “Metabolism and Membrane Permeability of Erythrocytes and Thrombocytes”. Stuttgart, Verlag, pp 15.Google Scholar
  17. Williamson, J. (1970) in Brewer, G.J., ed. “Red Cell Metabolism and Function”. Plenum Press, New York, pp 117.Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • Michael C. Brain
    • 1
  • Robert T. Card
    • 1
  1. 1.Department of MedicineMcMaster University HamiltonHamiltonCanada

Personalised recommendations