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The Isolation and Characterization of Synaptosomal Plasma Membranes

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Glycolipids, Glycoproteins, and Mucopolysaccharides of the Nervous System

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 25))

Abstract

The importance of methods for the isolation of fractions enriched in neuronal material, synaptosomal fractions (1, 2), synaptic vesicles (3, 5) and synaptosomal plasma membranes (3, 4, 6) can be seen from the large amount of work which has been published on their properties (for review, see 7–10). Unfortunately, despite the proliferation of papers in this field, these fractions have never been thoroughly characterized enzymatically and chemically. We therefore decided to attempt to isolate one of these fractions, the synaptosomal plasma membrane, in a form suitable for further study of its composition.

This work has been, in part, supported by grants from the Institut National de la Santé et de la Recherche Médicale (Contract 71 11698) and the Fondation pour la Recherche Médicale Française.

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Author notes

  1. M. Reith

    Present address: Rudolf Magnus Institute, Utrecht, The Netherlands

  2. U. Marinari

    Present address: Istituto di Patologia Generale, Università di Genova, Italy

Authors and Affiliations

  1. Faculté de Médecine, Centre de Neurochimie du CNRS and Institut de Chimie Biologique, 67 — Strasbourg, France

    I. G. Morgan, M. Reith, U. Marinari, W. C. Breckenridge (Fellow) & G. Gombos (Attaché de Recherche)

  2. Boursier étranger de l’Institut National de la Santé et de la Recherche Médicale, France

    I. G. Morgan

  3. Medical Research Council of Canada, Canada

    W. C. Breckenridge (Fellow)

  4. Centre National de la Recherche Scientifique, France

    G. Gombos (Attaché de Recherche)

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  1. I. G. Morgan
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  4. W. C. Breckenridge
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Editors and Affiliations

  1. Institute of Biological Chemistry Medical School, University of Milan, Milan, Italy

    Vittorio Zambotti (Symposium Chairman Head), Guido Tettamanti (Professor of Biochemistry) & Mariagrazia Arrigoni (Symposium Chairman Head),  (Professor of Biochemistry) & 

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© 1972 Plenum Press, New York

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Morgan, I.G., Reith, M., Marinari, U., Breckenridge, W.C., Gombos, G. (1972). The Isolation and Characterization of Synaptosomal Plasma Membranes. In: Zambotti, V., Tettamanti, G., Arrigoni, M. (eds) Glycolipids, Glycoproteins, and Mucopolysaccharides of the Nervous System. Advances in Experimental Medicine and Biology, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3216-9_13

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  • DOI: https://doi.org/10.1007/978-1-4684-3216-9_13

  • Publisher Name: Springer, Boston, MA

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