In Vitro Studies of Delayed Hypersensitivity by the Method of Macrophage Spreading Inhibition.Delayed Hypersensitivity to Tuberculin and Diphtheria Toxoid in Rats

  • D. Dekaris
  • Blanka Veselić
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 15)


It was shown by early experiments (1) in mice or guinea pigs having delayed hypersensitivity, that the ability of peritoneal macrophages to spread is markedly decreased by the presence of specific antigen. The spreading inhibition can be easily determined and used as a simple in vitro test for the study of delayed hypersensitivity. The method was named macrophage spreading inhibition (MSI) and is usefully applied for the detection of delayed hypersensitivity to bacterial and protozoal antigens in guinea pigs and mice (1,2,3,4).


Peritoneal Macrophage Intradermal Injection Cutaneous Reactivity Peritoneal Cell Diphtheria Toxoid 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Fauve, R.M. and D. Dekaris, Science, 160:795, 1968.PubMedCrossRefGoogle Scholar
  2. 2.
    Dekaris, D., R.M. Fauve and M. Raynaud, J. Immunol, 103: 1, 1969.PubMedGoogle Scholar
  3. 3.
    Dekaris, D. and B. Veselić, Period. biol, 72:69, 1970.Google Scholar
  4. 4.
    Seah, S., Nature, 225:1256, 1970.PubMedCrossRefGoogle Scholar
  5. 5.
    Dekaris, D., B. Veselić and V. Tomažič, Immunology, in press.Google Scholar
  6. 6.
    Brogan T.D., this volume.Google Scholar

Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • D. Dekaris
    • 1
  • Blanka Veselić
    • 1
  1. 1.“Rudjer Bošković” InstituteZagrebYugoslavia

Personalised recommendations