Partial Purification of Rat Urinary Kininogenase and its Reactions with Active Center Reagents of Trypsin
The kininogenase from rat urine has the properties of a trypsin-like enzyme, as determined with the help of synthetic substrates or competitive inhibitors (Mares-Guia and Diniz, 1967). The work done previously was carried out with a partially purified preparation of high specific activity. The purification procedure will now be described, as well as the behavior of the enzyme in the presence of specific reagents for the trypsin active center.
KeywordsMethylene Blue Active Center Ethyl Ester Amidase Activity Chloromethyl Ketone
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