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Metabolism of 14C-Bradykinin in Man

  • Harry R. Keiser
  • Marjorie P. Peyton
  • John J. Pisano
  • Albert Sjoerdsma
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 8)

Abstract

Although kinins have been implicated in a variety of physiological and pathological conditions, often definitive proof of their involvement has been lacking. This situation exists because: 1) specific blockers of kinin liberation or utilization are not available, 2) kinins and related enzymes and proteins are difficult to measure, and 3) there are no known metabolites of kinins. In an attempt to get at some of the problems, the National Heart Institute has financed the synthesis of radioactive kinins and made them available to qualified researchers (1,2). The radioactive peptides have been invaluable in the development of a radioimmunoassay for bradykinin (3,4), the elucidation of a proteolytic mechanism of kinin inactivation in lung (5), the characterization of kinins in human urine (6), and, as reported here, study of the kinetics of kinin metabolism and characterization of urinary metabolites in man.

Keywords

Human Urine Total Radioactivity Urinary Metabolite Appearance Time Malignant Carcinoid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pisano, J.J.: Radioactive peptide available. Science 163: 494, 1969.PubMedCrossRefGoogle Scholar
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    Pisano, J. J.: Carbon-14 labeled vasoactive peptides available. Science 165: 203, 1969.PubMedCrossRefGoogle Scholar
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    Webster, M.E., Pierce, J.V. and Sampaio, M.U.: Studies on antibody to bradykinin. This volume.Google Scholar
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    Fischer, J., Spragg, J., Talamo, R.C., Pierce, J.V., Suzuki, K., Austen, K.F., and Haber, E.: Structural requirements for binding of bradykinin to antibody. III. The effect of carrier on antibody specificity. Biochemistry 8: 3750, 1969.PubMedCrossRefGoogle Scholar
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    Ryan, J.W., Roblero, J., and Stewart, J.M.: Inactivation of bradykinin in rat lung. This volume.Google Scholar
  6. 6.
    Miwa, Q., Erdös, E.G., and Seki, T.: Presence of three peptides in urinary kinin (substance Z) preparations. Life Sciences 7: 1339–1343, 1968.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Harry R. Keiser
    • 1
  • Marjorie P. Peyton
    • 1
  • John J. Pisano
    • 1
  • Albert Sjoerdsma
    • 1
  1. 1.Section of Experimental Medicine and the Section of Physiological Chemistry, Experimental Therapeutics BranchNational Heart InstituteBethesdaUSA

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