Response of the Circulatory System to Bradykinin and Related Peptides: A Specific Kinin Assay

  • John M. Stewart
  • Juan Roblero
  • James W. Ryan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 8)


The complex response of the mammalian circulatory system to bradykinin and related peptides is made up both of direct actions of the peptides as well as responses of the organism which tend to neutralize the actions of the kinins. The direct vasomotor actions of bradykinin are arteriolar dilation, venular constriction, and increased capillary permeability. These cause the observed sharp fall in blood pressure. Catecholamine release, tachyphylaxis, and increased cardiac output partially neutralize the direct effects, and a very efficient multienzyme kinin metabolizing system rapidly removes bradykinin from the circulation. To study these effects we have used synthetic peptides related in structure to the kinins, in addition to certain drugs. The peptides were synthesized by the solid phase method (Stewart and Young, 1969). An adequate understanding of the factors involved has made it possible to develop a rat blood pressure assay which is specific for each of the known mammalian kinins.


Related Peptide Catecholamine Release Solid Phase Method Arteriolar Dilation Blood Pressure Study 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. E. G. Erdos (1966). Adv. Pharmacol. 4, 1.PubMedCrossRefGoogle Scholar
  2. S. H. Ferreira and J. R. Vane (1967). Brit. J. Pharmacol. Chemother. 30, 417.Google Scholar
  3. L. M. Greenbaum, R. Freer, G. Semente and K. Yamafuji (1969). Brit. J. Pharmacol. Chemother-,in press.Google Scholar
  4. L. J. Greene, J. M. Stewart and S. H. Ferreira (1969). This volumeGoogle Scholar
  5. J. W. Ryan, J. Roblero and J. M. Stewart (1968). Bîochem. J. 110, 795.PubMedGoogle Scholar
  6. J. W. Ryan, J. Roblero and J. M. Stewart (1969). This volumeGoogle Scholar
  7. E. Schroder and K. Lubke (1966). “The Peptides”. Academic Press, New York, Vol 2, p. 109.Google Scholar
  8. J. M. Stewart (1968a). Federation Proc. 27, 63.Google Scholar
  9. J. M. Stewart (1968b). Federation Proc. 27, 534.Google Scholar
  10. J. M. Stewart and J. Roblero (1967). “Vasoactive Polypeptides and Inhibitors of Proteolytic Enzymes”. Bayer Yakuhin K. K., Tokyo, p. 52.Google Scholar
  11. J. M. Stewart and D. W. Wool ley (1965). Nature 207, 1160.PubMedCrossRefGoogle Scholar
  12. J. M. Stewart and J. D. Young (1969). “Solid Phase Peptide Synthesis”. W. H. Freeman, San Francisco.Google Scholar

Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • John M. Stewart
    • 1
  • Juan Roblero
    • 2
  • James W. Ryan
    • 3
  1. 1.University of Colorado Medical SchoolDenverUSA
  2. 2.Universidad Catolica de ChileSantiagoChile
  3. 3.University of Miami School of MedicineMiamiUSA

Personalised recommendations