Dependence of Oxyhemoglobin Dissociation and Intra-Erythrocytic 2.3-DPG on Acid-Base Status of Blood. I. In Vitro Studies on Reduced and Oxygenated Blood

Abstract

According to the conception of the regulation of the intraerythrocytic 2.3-DPG concentration, introduced recently, the most important regulatory factor should be the level of reduced hemoglobin in the erythrocytes (i.e.either the “average” level in the circulatory system or the maximal level attained on the venous side) (GARBY AND DE VERDIER, 1969, BENESCH AND BENESCH, 1969). The main support for this hypothesis comes from the well-known increase in 2.3-DPG concentration seen under several hypoxic conditions (LENFANT ET AL.,1968). The adaptional nature of this regulatory mechanism should be imitable in vitro under proper incubation conditions. This paper describes the results of some in vitro experiments, where we have studied the effect of pH, deoxygenation and addition of different substances to human blood.