Biological Alterations Occurring During Red Cell Preservation
Removal of the red blood cell from the normal circulation drastically alters its external environment, depriving the cell of necessary nutrients as well as removing certain protective factors. To counter the loss of nutrient, various blood collecting solutions have been devised in which glucose is used to supply the cell with an energy source. At the same time, attempts are made to reduce cellular metabolism, hence cell demand for energy, usually by using solutions of low pH and/or mechanical factors, that of cooling the blood. The combination of a slower metabolism and a glucose supplement have permitted most storage of red cells in a useful state for perhaps 20–30 days (1,2,3).
KeywordsMechanical Agitation Osmotic Fragility Free Hemoglobin Blood Storage Oxygen Function
Unable to display preview. Download preview PDF.
- 1.Mollison, P.L.: Blood Transfusion in Clinical Medicine. Philadelphia, F.A. Davis Co., 4th ed., 1967.Google Scholar
- 2.Strumia, M.M., W. H. Crosby, J. G. Gibson, Ii, T. J. Greenwalt, and J. R. Krevans: General Principles of Blood Transfusion. From Transfusion, by National Academy of Science, 1963.Google Scholar
- 7.Sugita, Y., and A. Chanutin: Electrophoretic studies of red cell hemolysates supplemented with phosphorylated carbohydrate intermediates. Proc. Soc. Exp. Bio. 112:72, 1963.Google Scholar
- 10.Ham, T. H., S. C. Shen, E. M. Fleming, and W. B. Castle: Studies on the destruction of red blood cells. IV. Thermal injury: Action of heat in causing increased spheroidicity, osmotic and mechanical fragilities and hemolysis of erythrocytes in dogs and in a patient with a fatal thermal burn. Blood, 3:373, 1948.PubMedGoogle Scholar
- 12.Dawson, R.B., Jr., W. F. Kocholaty, C. E. Shields, T. J. Ellis, and E. Bowles-Ledford: The hemoglobin function of blood stored at 4°C. USAMRL Lab. Rept., in press.Google Scholar