Biological Alterations Occurring During Red Cell Preservation

  • Charles E. Shields
  • Harold S. Kaplan
  • Roy B. DawsonJr.
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 6)


Removal of the red blood cell from the normal circulation drastically alters its external environment, depriving the cell of necessary nutrients as well as removing certain protective factors. To counter the loss of nutrient, various blood collecting solutions have been devised in which glucose is used to supply the cell with an energy source. At the same time, attempts are made to reduce cellular metabolism, hence cell demand for energy, usually by using solutions of low pH and/or mechanical factors, that of cooling the blood. The combination of a slower metabolism and a glucose supplement have permitted most storage of red cells in a useful state for perhaps 20–30 days (1,2,3).


Mechanical Agitation Osmotic Fragility Free Hemoglobin Blood Storage Oxygen Function 
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Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Charles E. Shields
    • 1
  • Harold S. Kaplan
    • 1
    • 2
  • Roy B. DawsonJr.
    • 1
  1. 1.Blood Transfusion DivisionUS Army Medical Research LaboratoryFort KnoxUSA
  2. 2.Washington University School of MedicineSt. LouisUSA

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