In Vitro Reactivation of Antibody Response in Explants of Irradiated Spleens

  • Amiela Globerson
  • Michael Feldman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 5)


The induction of antibody formation to quite a number of antigens appears to involve a bicellular mechanism of macrophage-lymphocyte interaction. Fishman [1] demonstrated that lymphocytes could be triggered to produce antibody in vitro when treated with macrophages that had interacted with antigen. This observation was followed by further claims regarding the involvement of macrophages in the production of antibody to protein antigens [2], bacterial antigens [3], and red blood cells [4]. The macrophage-antigen interaction was shown to contribute an essential step in the induction of antibody production to Shigella antigens [3, 5]. Mice exposed to sublethal doses of total-body X-irradiation failed to produce antibodies when injected with Shigella alone. Such mice did, however, produce specific agglutinating antibody following the inoculation of macrophages from normal donors that interacted in vitro with Shigella antigen. Evidence was obtained to indicate that in this system antigen-treated macrophages triggered the production of antibody by lymphocytes of the irradiated recipients [5].


Antibody Response Sublethal Dose Peritoneal Exudate Thymus Tissue Chick Embryo Extract 
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Copyright information

© Plenum Press 1969

Authors and Affiliations

  • Amiela Globerson
    • 1
  • Michael Feldman
    • 1
  1. 1.Department of Cell BiologyThe Weizmann Institute of ScienceRehovothIsrael

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