Alloantibody-Forming Cells in Skin Allografted Mice Detected by Immunocytoadherence

  • H. S. Micklem
  • N. A. Staines
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 5)


Previous work [1, 2] has shown that the immune response to skin allografts in mice is biphasic. Before rejection of the graft cellular proliferation in the regional lymph nodes is largely confined to the zone variously termed “intermediate” [3], “paracortical” [4], or “thymus-dependent” [5]; little or no activity is detectable in the outer cortical zone or in the medulla, nor is antibody detectable in the serum. At the time of rejection, proliferative activity switches to the outer cortex, where germinal centers rapidly enlarge, and to the medulla, where plasmacytes become numerous. At the same time hemagglutinating and cytotoxic antibodies appear in the serum. Sublethal X-irradiation of the host immediately before grafting was found to suppress the second phase of the response while only marginally affecting the first phase and the time of rejection of the graft. These results implied that antibody formation, associated with cell proliferation in the germinal centers and medulla, was a consequence rather than a cause of graft rejection [6]. However, it remained possible that antibody was formed before graft rejection but was undetected either because it was not released from the manufacturing cells or because it was absorbed by the graft. Thanks to the development of methods for the detection of antibody production by single cells [7, 8, 9, 10], further investigation has now been possible.


Germinal Center Graft Rejection Irradiate Mouse Cytotoxic Antibody Total White Cell Count 
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  1. 1.
    H. S. Micklem and J. A. H. Brown, Immunology, 4:318, 1961.PubMedGoogle Scholar
  2. 2.
    H. S. Micklem and J. A. H. Brown, in: H. Cottier, N. Odartchenko, R. Schindler, and C. C Congdon, Eds., Germinal Centers in Immune Responses, p. 277. New York: Springer-Verlag, 1967.CrossRefGoogle Scholar
  3. 3.
    T. B. Dunn, J. Natl. Cancer Inst., 14:1281, 1954.PubMedGoogle Scholar
  4. 4.
    J. Oort and J. L. Turk, Brit. J. Exptl. Pathol., 46:147, 1965.Google Scholar
  5. 5.
    D. M. V. Parrott, M. A. B. de Sousa, and J. East, J. Exptl. Med., 123:191, 1966.CrossRefGoogle Scholar
  6. 6.
    H. S. Micklem and J. F. Loutit, Tissue Grafting and Radiation, chapter 3. New York: Academic Press, 1966.Google Scholar
  7. 7.
    G. Biozzi, C. Stiffel, D. Mouton, M. Lacopoulos-Briot, C. Decreusefond, and Y. Bouthillier, Ann. Inst. Pasteur, 110:7, 1966.Google Scholar
  8. 8.
    G. Biozzi, C. Stiffel, D. Mouton, Y. Bouthillier, and C. Decreusefond, Immunology, 14:7, 1968.PubMedGoogle Scholar
  9. 9.
    O. B. Zaalberg, V. A. van der Meul, and M. J. van Twisk, Nature, 210:544, 1966.PubMedCrossRefGoogle Scholar
  10. 10.
    O. B. Zaalberg, V. A. van der Meul, and M. J. van Twisk, J. Immunol., 100:451, 1968.PubMedGoogle Scholar
  11. 11.
    P. A. Gorer and Z. B. Mikulska, Cancer Res., 14:651, 1954.PubMedGoogle Scholar
  12. 12.
    G. A. Voisin and R. G. Kinsky, in: G. E. Wolstenholme and M. P. Cameron, Eds., Ciba Foundation Symposium, p. 286. London: J. and A. Churchill, 1962.Google Scholar
  13. 13.
    W. H. Hildemann, Transplantation, 5:1001, 1967.PubMedCrossRefGoogle Scholar
  14. 14.
    D. Y. Perey, M. D. Cooper, and R. A. Good, Transplantation, 5:615, 1967.PubMedCrossRefGoogle Scholar
  15. 15.
    H. Gewürz, D. S. Clark, J. Finstad, W. D. Kelly, R. L. Varco, R. A. Good, and A. E. Gabrielson, Ann. N.Y. Acad. Sci., 129:573, 1966.Google Scholar
  16. 16.
    U. Rother, D. L. Ballantyne, Jr., C. Cohen, and K. Rother, J. Exptl. Med., 126:656, 1967.CrossRefGoogle Scholar
  17. 17.
    C. A. Stetson, Advan. Immunol., 3:97, 1963.CrossRefGoogle Scholar
  18. 18.
    G. Möller, J. Immunol., 96:430, 1966.PubMedGoogle Scholar
  19. 19.
    B. Andersson, H. Wigzell, and G. Klein, Transplantation, 5:11, 1966.CrossRefGoogle Scholar
  20. 20.
    M. A. Mandel, and R. Asofsky, J. Immunol., 100:363, 1968.PubMedGoogle Scholar
  21. 21.
    A. J. S. Davies, E. Leuchars, V. Wallis, P. Marchant, and E. V. Elliot, Transplantation, 5:222, 1967.CrossRefGoogle Scholar
  22. 22.
    A. J. S. Davies, E. Leuchars, V. Wallis, and P. C. Koller, Transplantation, 4:438, 1966.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press 1969

Authors and Affiliations

  • H. S. Micklem
    • 1
  • N. A. Staines
    • 1
  1. 1.Immunobiology UnitDepartment of ZoologyEdinburgh, 9Scotland

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