Alloantibody-Forming Cells in Skin Allografted Mice Detected by Immunocytoadherence
Previous work [1, 2] has shown that the immune response to skin allografts in mice is biphasic. Before rejection of the graft cellular proliferation in the regional lymph nodes is largely confined to the zone variously termed “intermediate” , “paracortical” , or “thymus-dependent” ; little or no activity is detectable in the outer cortical zone or in the medulla, nor is antibody detectable in the serum. At the time of rejection, proliferative activity switches to the outer cortex, where germinal centers rapidly enlarge, and to the medulla, where plasmacytes become numerous. At the same time hemagglutinating and cytotoxic antibodies appear in the serum. Sublethal X-irradiation of the host immediately before grafting was found to suppress the second phase of the response while only marginally affecting the first phase and the time of rejection of the graft. These results implied that antibody formation, associated with cell proliferation in the germinal centers and medulla, was a consequence rather than a cause of graft rejection . However, it remained possible that antibody was formed before graft rejection but was undetected either because it was not released from the manufacturing cells or because it was absorbed by the graft. Thanks to the development of methods for the detection of antibody production by single cells [7, 8, 9, 10], further investigation has now been possible.
KeywordsGerminal Center Graft Rejection Irradiate Mouse Cytotoxic Antibody Total White Cell Count
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