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Mouse Blood Lymphocyte Origins Investigated by a Simple Cell Culture Technique

  • H. Festenstein
  • A. J. S. Davies
  • E. Leuchars
  • V. J. Wallis
  • M. J. Doenhoff
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 5)

Abstract

The thymus may affect the maturation of immunologically active cells mainly, or solely, by the liberation of some humoral factor which is active external to the thymus [1]; it may alternatively do so by the release of immunologically competent lymphocytes actually arising in the thymus or by the exertion of a maturing effect on cells migrating through the thymus [2]. These problems are of considerable importance to the understanding of many basic immunological phenomena and have therapeutic implications. No one has yet succeeded in isolating a thymus humoral factor. On the other hand, evidence that the thymus not only liberates cells which are seeded to spleen and lymph node but also that these thymus-derived lymphocytes are functionally, although not morphologically, distinguishable has been presented [3, 4]. In this study the question was asked: Do cells which originate in the thymus find their way into peripheral blood? It became possible to attempt to answer this question because of the development of Festenstein’s technique of the culture of mouse lymphocytes [5, 6].

Keywords

International Atomic Energy Agency Mixed Culture Peripheral Blood Lymphocyte Mouse Lymphocyte Allogeneic Lymphocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    D. Osoba and J. F. A. P. Miller, J. Exptl. Med., 119:177, 1964.CrossRefGoogle Scholar
  2. 2.
    J. E. Harris, C. E. Ford, D. W. H. Barnes and E. P. Evans, Nature, 201:884, 1964.PubMedCrossRefGoogle Scholar
  3. 3.
    E. Leuchars, A. M. Cross, A. J. S. Davies and V. J. Wallis, Nature, 203:1189, 1964.PubMedCrossRefGoogle Scholar
  4. 4.
    A. J. S. Davies, E. Leuchars, V. J. Wallis and P. C. Koller, Transplantation, 4:438, 1966.PubMedCrossRefGoogle Scholar
  5. 5.
    H. Festenstein, Ann. N. Y. Acad. Sci., 129:567, 1966.CrossRefGoogle Scholar
  6. 6.
    H. Festenstein, Lancet, i:182, 1968.CrossRefGoogle Scholar

Copyright information

© Plenum Press 1969

Authors and Affiliations

  • H. Festenstein
    • 1
    • 2
  • A. J. S. Davies
    • 1
    • 2
  • E. Leuchars
    • 1
    • 2
  • V. J. Wallis
    • 1
    • 2
  • M. J. Doenhoff
    • 1
    • 2
  1. 1.Transplantation Immunology UnitLondon Hospital Medical CollegeLondonE.l.
  2. 2.Chester Beatty Research Institute, Institute of Cancer ResearchRoyal Cancer HospitalLondon, S. W. 3England

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