Biochemical Identification of Membrane Receptors: Principles and Techniques

  • M. D. Hollenberg
  • P. Cuatrecasas
Part of the Handbook of Psychopharmacology book series (HBKPS, volume 2)


Since the earliest pharmacological studies, the cell membrane has been considered a likely site of drug interaction (Clark, 1933). Yet only within the past decade has considerable progress been made in the identification and purification of a variety of membrane-localized hormone receptors. This chapter will not attempt a comprehensive summary of all such studies completed to date; rather, an attempt will be made to elaborate on a few specific studies which, it is felt, will not only illustrate the general approaches that can be used to study ligand-membrane interactions but will also reveal some of the successes and pitfalls of these approaches. Examples will be drawn largely from work done in this laboratory, primarily because of our familiarity with the intimate details of these studies, both published and unpublished. Furthermore, examples will deal only with several of the polypeptide hormones and with catecholamines and will thus omit a large body of work on cholinergic receptors and on membrane receptors for an enlarging number of polypeptide hormones and other compounds. Studies on cytoplasmic for steroids are considered beyond the scope of this discussion. For details of these studies, the reader is referred to review articles elsewhere (Hall, 1972; Cuatrecasas, 1973a, 1974; Lis and Sharon. 1973; Hollenberg and Cuatrecasas, 1975a).


Insulin Receptor Membrane Receptor Wheat Germ Agglutinin Insulin Binding Biochemical Identification 


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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • M. D. Hollenberg
    • 1
    • 2
  • P. Cuatrecasas
    • 1
    • 2
  1. 1.Department of Pharmacology and Experimental TherapeuticsThe Johns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Department of MedicineThe Johns Hopkins University School of MedicineBaltimoreUSA

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