Mössbauer Studies of Vitamin B12 and Some Related Cobalamins

  • R. T. Mullen


The Mössbauer spectra of vitamin B12-Co57 (cyanocobalamin-Co57), dicyanocobalamin, and several aquocobalamin derivatives have been investigated. Sources were prepared as thin poly crystalline solids or frozen aqueous solutions and run, mainly at 77°K, against a Na4Fe(CN)6 • 10H2O absorber. The spectra of these cobalamins consist in general of a fairly strong, broad (~ 1 mm/sec half-width) doublet with evidence in many, but not all, cases of a minor contribution from a second doublet. Quadrupole splittings vary from 1.8 to 3.1 mm/sec; isomer shifts (vs. SNP) are largely in the +0.25–0.55 mm/sec range. Chemical changes in dicyanocobalamin solutions are observable by Mössbauer spectroscopy, although undetected by ultraviolet spectroscopy. The similarity of the Mössbauer spectrum of vitamin B12 to those of certain other biologically active compounds, particularly cytochrome c, is discussed, as is the general problem of interpreting “source” experiments.


Mossbauer Spectroscopy Isomer Shift Quadrupole Splitting Mossbauer Spectrum Cyanide Anion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    U. Gonser, R. W. Grant, and J. Kregzde, Science 143:680 (1964).CrossRefGoogle Scholar
  2. 2.
    Y. S. Moshkovskii, “Applications of the Mössbauer Effect in Biology,” in: Chemical Applications of Mössbauer Spectroscopy, V. I. Goldanskii and R. M. Herber, eds. (Academic Press, New York, 1968), Chap. 10.Google Scholar
  3. 3.
    R. Cooke and P. Debrunner, J. Chem. Phys. 48:4532 (1968).CrossRefGoogle Scholar
  4. 4.
    A. Nath, M. Harpold, M. P. Klein, and W. Kündig, Chem. Phys. Letters 2:471 (1968).CrossRefGoogle Scholar
  5. 5.
    E. L. Smith, Vitamin B 12 (John Wiley & Sons, New York, 1965), Chap. 4.Google Scholar
  6. 6.
    E. Kankeleit, Rev. Sci. Instr. 35:194 (1964).CrossRefGoogle Scholar
  7. 7.
    R. L. Cohen, P. G. McMullin, and G. K. Wertheim, Rev. Sci. Instr. 34:671 (1963).CrossRefGoogle Scholar
  8. 8.
    G. H. Beaven, E. R. Holiday, E. A. Johnson, B. Ellis, and V. Petrow, J. Pharm. Pharmacol. 2:944 (1950).CrossRefGoogle Scholar
  9. 9.
    J. B. Conn, S. L. Norman, and T. G. Wartman, Science 113:658 (1951).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • R. T. Mullen
    • 1
  1. 1.Radioactivity Laboratory, Merck Sharp & Dohme Research LaboratoriesDivision of Merck & Co., Inc.RahwayUSA

Personalised recommendations