Identification of Beta-Lactamases by Analytical Isoelectric Focusing
Analytical isoelectric focusin gives a direct visual comparison of beta-lactamases, down to extremely low levels of activity, from very crude intracellular preparations. It differentiates between enzymes that are biochemically and immunologically indistinguishable. The genus of the host strain does not affect the isoelectric focusing pattern of an R-factor-mediated beta-lactamase. This can therefore be used in epidemiological studies to monitor the transfer of resistance. Chromosomal beta-lactamases appear to be genus, species and subspecies specific; separation of the enzymes by isoelectric focusion could therefore be used as an aid to taxonomic grouping.
KeywordsTaxonomic Grouping Substrate Profile Klebsiella Aerogenes Genus Klebsiella Adjacent Track
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