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Identification of Beta-Lactamases by Analytical Isoelectric Focusing

  • M. Matthew
  • A. M. Harris
Part of the Chemotherapy book series (CT, volume 5)

Summary

Analytical isoelectric focusin gives a direct visual comparison of beta-lactamases, down to extremely low levels of activity, from very crude intracellular preparations. It differentiates between enzymes that are biochemically and immunologically indistinguishable. The genus of the host strain does not affect the isoelectric focusing pattern of an R-factor-mediated beta-lactamase. This can therefore be used in epidemiological studies to monitor the transfer of resistance. Chromosomal beta-lactamases appear to be genus, species and subspecies specific; separation of the enzymes by isoelectric focusion could therefore be used as an aid to taxonomic grouping.

Keywords

Taxonomic Grouping Substrate Profile Klebsiella Aerogenes Genus Klebsiella Adjacent Track 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • M. Matthew
    • 1
  • A. M. Harris
    • 1
  1. 1.Microbiology DepartmentGlaxo Research Ltd.Greenford, Middx.UK

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