Advertisement

Structure-Activity Study on the Kasugamycin Family

  • Makoto Hori
  • Kayoko Suzukake
  • Yoshimasa Uehara
  • Hamao Umezawa
Part of the Chemotherapy book series (CT, volume 3)

Abstract

Minosaminomycin (MSM), which is structurally related to kasugamycin (KSM), is active against mycobacteria but not against Gram negative bacteria. In vivo studies showed that MSM inhibits protein synthesis in mycobacteria. MSM, at 2×10−7M, also inhibits f2 RNA-directed protein synthesis in an E. coli cell-free system by 50%. This inhibition is 100 times stronger than that caused by KSM in the same system. Like KSM, MSM preferentially inhibits the initiation process of protein synthesis. No increase in miscoding is observed in the presence of MSM. KG-8, a chemical derivative of KSM, is 10 times more inhibitory to the growth of E. coli than KSM. However, in the in vitro protein synthesizing system, KG-8 is not as inhibitory as KSM. These discrepancies between in vivo and in vitro activities may be ascribed to a difference in permeability, since E. coli cells with modified permeability show altered sensitivity to MSM. There is no indication that MSM is inactivated in E. coli cells. Based on these findings, the structure essential for binding to ribosomes and for permeation are discussed.

Keywords

Pyruvate Kinase Negative Bacterium Initiation Process Chemical Derivative Potent Antimicrobial Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gros, F. et al. (1967), J. Mol. Biol., 25, 555.PubMedCrossRefGoogle Scholar
  2. Hamada, M. et al. (1974), J. Antibiot., 27, 81.PubMedCrossRefGoogle Scholar
  3. Okuyama, A. et al. (1974), Biochem. Biophys. Res. Comm., 60, 1163.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Makoto Hori
    • 1
  • Kayoko Suzukake
    • 1
  • Yoshimasa Uehara
    • 1
  • Hamao Umezawa
    • 1
  1. 1.Institute of Microbial ChemistryShinagawa-ku, TokyoJapan

Personalised recommendations