14C-Phenyl-Alanine Incorporation in the Presence of Veral Antibiotics on B. subtilis Transfection
Using a system for competence stimulation in B. subtilis six inhibitors of protein synthesis were tested through the 14C-phenyl-alanine incorporation, cell survival and competence. When a 20% mortality is produced, independently of the doses, gentamycin and lincomycin were the most active drugs. However, in absolute comparisons, tetracyclin molecules presented the maximal activity in such a sensitive biological system.
KeywordsMinimal Salt Medium Competence Development Transfection Procedure Serial Dilution Technique Absolute Comparison
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