Methods in Microsporidiology

  • Jiří Vávra
  • J. V. Maddox
Part of the Comparative Pathobiology book series (CPATH, volume 1)


The purpose of this chapter is to inform the reader (at all levels of experience with microsporidian research) of the methods most commonly used by microsporidiologists. In addition to the classical methods that have been used for many years, more sophisticated techniques, utilizing many different scientific disciplines, are increasingly being employed in the study of microsporidia. We believe that a working knowledge of this methodology should be possessed by workers in the field of microsporidology. We realize that old methods are constantly being modified and new methods developed. Consequently, this chapter will be out of date soon after it is finished. In any event, we hope it will help microsporidologists expand the scope of their research.


Spore Suspension Cover Slip Mosquito Larva Indirect Fluorescent Antibody Test Polar Filament 
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  1. Abdel-Malek, A., and Steinhaus, A, (1988). Invasion route of No8ema sp. in the potato tuberworm, as determined by ligaturing. J. Parasitol. 34Google Scholar
  2. Alger, N. (1966). A simple, rapid, precise stain for intestinal protozoa. Amer. J. Clin. Pathol. 19, 361–362.Google Scholar
  3. Allen, H. V., and Brunson, M. H. (1977) Control of Nosema disease of potato tuberworms, a host used in the mass production of Macrocentrus ancylivorus. Science 105: 39Google Scholar
  4. Angus, T. A. (1964). A magnetic stirring device for syringes. J. Invertebr. Pathol. 6: 126.Google Scholar
  5. Bailey, L. (1972). The preservation of infective microsporidian spores. J. Invertebr. Pathol. 20: 252–251.CrossRefGoogle Scholar
  6. Bedrnik, P., and Vavra, J. (1971). Cryopreservation of the mammalian microsporidian Nosema cimiculi. J. Protozool. 18 (Suppl.): 9.Google Scholar
  7. Bedrnik, P., and Vavra, J. (1972). Further observations on the maintenance of Encephalitozoon cwiculi in tissue culture. J. Protozool. 19 (Suppl.): 75.Google Scholar
  8. Bismanis, J. E. (1970). Detection of latent murine nosematosis and growth of Nosema cimiculi in cell cultures. Canad. J. Microbiol. 16: 237–242.CrossRefGoogle Scholar
  9. Burges, H. D., Canning, E. U., and Hurst, J. A. (1971). Morphology development, and pathogenicity of Nosema oryzoephili N. sp. in Oryzaephibes surinamensis and its host range among granivorous insects. J. Invertebr. Pathol. 17: 319–332.Google Scholar
  10. Burges, H. D., and Thompson, E. M. (1971). Standardization and assay of microbial insecticides. In “Microbial Control of Insects and Mites” ( H. D. Burges and N. W. Hussey, eds.). Academic Press, New York.Google Scholar
  11. Canning, E. U., and Hulls, R. (1970). A microsporidian infection of Anopheles gambia Gibs, from Tanzania, interpretation of its mode of transmission and notes on Nosema infections in mosquitoes. Protozool. 17: 531–539CrossRefGoogle Scholar
  12. Cerkasovova, A., and Vavra, J. (1972). Disintegration of microsporidian spores for physiological studies. SIP Newsletter 4: 21.Google Scholar
  13. Chalupsky, J., Bedrnik, P., and Vavra, J. (1971). The indirect fluorescent antibody test for Nosema cimiculi. J. Protozool. 18 (Suppl.): 107.Google Scholar
  14. Chalupsky, J., Vavra, J., and Bedrnik, P. (1973). Detection of antibodies to Encephalitozoon cimiculi in rabbits by the indirect immunofluorescent antibody test. Folia Parasitol. (Praha) 20: 281–289.Google Scholar
  15. Cole, R. J. TT970). The application of the “triangulation” method to the purification of Nosema spores from insect tissues. J. Invertebr. Pathol. 15: 193–195.Google Scholar
  16. Conner, R. M. (1970). Disruption of microsporidian spores for serological studies. J. Invertebr. Pathol. 15: 138.CrossRefGoogle Scholar
  17. Cox, J. C., Waiden, N. B., and Nairn, R. C. (1972). Presumptive diagnosis of Nosema cuniculi in rabbits by immunofluorescence. Res. Vet. Sei. 13: 595–597.Google Scholar
  18. Fischer, F. M., and Sanborn, R. C. (1959). Pathogenicity of a sporozoan parasite analyzed by tissue culture. Abstract Fed. Amer. Soc. Expt. Biol. Proc. 18: 85.Google Scholar
  19. Fischer, F. M. (1961). Interactions between a sporozoan and its insect hosts. Ph.D. thesis, Department of Zoology, Purdue University.Google Scholar
  20. Fowler, J., and Reeves, E. (1974). Detection of relationships among microsporidian isolates by electrophoretic analysis: Hydrophobic extracts. J. Invertebr. Pathol. 23: 3–12.PubMedCrossRefGoogle Scholar
  21. Fowler, J. and Reeves, E. (1974b). Detection of relationships among microsporidian isolates by electrophoretic analysis: Hydrophilic extracts. J. Invertebr. Pathol. 23: 63–69.PubMedCrossRefGoogle Scholar
  22. Fowler, J. L., and Reeves, E. L. (1974c). Spore dimorphism in a microsporidian isolate. J. Protozool. 21: 538 - 542.PubMedCrossRefGoogle Scholar
  23. Fritzsch, W. (1970). Erprobung eines Heilmittels gegen Nosematose. Arch. Exp. Vet. Med. 24: 951 - 984.Google Scholar
  24. Frost, S., and Nolan, R. A. (1972). The occurrence and morphology of Candospora spp. (Protozoa: Microsporida) in Newfoundland and Labrador blackfly larvae (Diptera: Simuliidae). Canad. J. Zool. 50: 1363–1366.CrossRefGoogle Scholar
  25. Fyg, W. (1963). Eine einfache Methode zur elektiven Färbung von Mikroorganismen in Ausstrichen und Gewebeschnitten. Z. Bienen.-Forsch. 6: 179–183.Google Scholar
  26. Gassouma, M.S.S., and Ellis, D. S. (1973). The ultrastructure of sporogonic stages and spores of Thelohania and Plistophora (Microsporida, Nosematidae) from Simulium ornatum larvae. J. Gen. Microbiol, 4: 33–43.Google Scholar
  27. Gontarski, H., and Wagner, O. (195). Quantitative Versuche zur chemotherapeutischen Bekämpfung von Nosema apis Z. bei der Honigbiene. Arzneim.-Forsch. 4: 161–168.Google Scholar
  28. Gude, W. D. (1968). Autoradiographic Techniques. Prentice-Hall., Inc. Englewood Cliffs, N. J. 113 pp.Google Scholar
  29. Henry, J. E. (1967). Nosema aoridophagus sp. N, a microsporidian isolated from grasshoppers. J. Invertebr. Pathol. 9: 331–341.CrossRefGoogle Scholar
  30. Henry, J. E. (1971). Experimental application of Nosema locustae for control of grasshoppers. J. Invertebr. Pathol. 18: 389–394.CrossRefGoogle Scholar
  31. Hink, W. F. (1972). A catalog of invertebrate cell lines. In Invertebrate Tissue Culture Volume Two, ( C. Vago ed.) Academic Press, New York.Google Scholar
  32. Hostounsky, Z. (1970). Nosema mesnili (Paill.), a microsporidian of the cabbage-worm, Pieris brassicae (L.) in the parasites Apanteles glomeratus (L.), Hyposoter ebenius (Grav.) and Pimpla instigator (F.). Acta. Entomol. Bohemoslov 7: 1–5.Google Scholar
  33. Hsiao, T. H., and Hsiao, C. (1973). Benomyl: a novel drug for controlling a microsporidian disease of the alfalfa weevil. J. Invertebr. Pathol. 22: 303–309.CrossRefGoogle Scholar
  34. Huger, A. (1960). Electron microscope study on the cytology of a microsporidian spore by means of ultrathin sectioning. J. Insect Pathol. 2: 81–105.Google Scholar
  35. Hunter, D. K. (1968). Response of populations of Chironomus californicus to a microsporidian (Gurleya sp.). J. Invertebr. Pathol. 10: 387–389.CrossRefGoogle Scholar
  36. Ignoffo, C. M., and Hink, W. F. (1971). Propagation of arthropod pathogens in living systems. In “Microbial Control of Insects and Mites” ( H. D. Burges and N. W. Hussey, eds.). Academic Press, New York.Google Scholar
  37. Innes, J.R.M., Zeman, W., Frenkel, J. K., and Borner, G. (1962). Occult endemic encephalitozoonosis of the central nervous system of mice (Swiss-Bagg-O’Grady strain). J. Neuropathol. Exp. Neurol. 21: 519–533.PubMedCrossRefGoogle Scholar
  38. Ishihara, Ren. (1998). Growth of Nosema bombycis in primary cell cultures of mammalian and chicken embryos. J. Invertebr. Pathol. 11: 328.CrossRefGoogle Scholar
  39. Ishihara, R., and Hayashi, Y. (1968). Some properties of ribosomes from the sporoplasm of Nosema bombycis. J. Invertebr. Pathol. 11: 377–385.CrossRefGoogle Scholar
  40. Ishihara, Ren, and Sohi, S. S. (1966). Infection of ovarian tissue cultures of Bombyx mori by Nosema bombycis spores. J. Invertebr. Pathol. 8: 538 - 540.CrossRefGoogle Scholar
  41. Jackson, S. J., Solorzano, R. F., and Middleton, C. C. (1973). An indirect fluorescent antibody test for antibodies to Nosema cimiculi (Encephalitozoon) in rabbits. Proc. Annual Meeting U.S. Animal Health Association 1973, 77th, pp. 478–490.Google Scholar
  42. Jenkins, J. N., McLaughlin, R. E., Parrott, W. L., and Wouters, C.J.J. (1970). Eliminating Glugea gasti (Protozoa: Microsporidia) from genetic stocks of the boll weevil. J. Econ.Google Scholar
  43. Entomol. 63: 1638–1639.Google Scholar
  44. Jirovec, O. (1932). Ergebnisse der Nuclealfarbung an den Sporen der Microsporidien nebst einigen Bemerkungen uber Lymphocystis. Arch. Protistenk 77: 379–390.Google Scholar
  45. Kalalova, S., and Weiser, J. (1973). Identification of microsporidia by indirect fluorescent antibody tests. Abst. Int. Conf. Insect Pathol., Fifth, Oxford, England; p. 111.Google Scholar
  46. Kaneda, Y. (1969). Studies on the effect of endoxan, an antitumor substance, to promote the growth of Nosema cuniculi in vivo and in vitro. Jap. J. Parasitol. 18: 291–303.Google Scholar
  47. Katznelson, H., and Jamieson, C. A. (1952). Control of Nosema disease of honey bees with fumagillin. Science 115: 70–71.PubMedCrossRefGoogle Scholar
  48. Kramer, J. P. (i960). Observations on the emergence of the microsporidian sporoplasm. J. Insect Pathol. 2: 133–139.Google Scholar
  49. Kramer, J, P. (1964). Nosema kingi sp. n., a microsporidian from Drosophila willistoni Sturtevant, and its infectivity for other muscoids. J. Insect Pathol. 6: 491–499.Google Scholar
  50. Kramer, J. P. (1965). Effect of an Ostosporeosis locomotor activity of adult Phormia regina (Meigen) (Dipt. Calliphoridae). Entomophaga 10: 339–342.CrossRefGoogle Scholar
  51. Kramer, J. P. (1998). An Octosporeosis of the black blowfly, Phormia regina: Incidence rates of host and parasite. A. fur Parasitenk 30: 33–39.CrossRefGoogle Scholar
  52. Kramer, J. P. (1970). Longevity of microsporidian spores with special reference to Octosporea muscaedomesticae Flu. Acta. Protozool. 8: 217–228.Google Scholar
  53. Kudo, R. (1921). Microsporidia Parasitic in Copepods. J. Parasitol. 7: 137–143.CrossRefGoogle Scholar
  54. Kudo, R. (1922). Studies on microsporidia parasitic in mosquitoes. II. On the effect of the parasite upon the host body. J. Parasitol. 8: 70–77.CrossRefGoogle Scholar
  55. Kudo, R. (192M. A biologic and taxonomic study of the Microsporidia. Illinois Biol. Monogr. IX, Nos. 1 and 2. 268 pp.Google Scholar
  56. Lainson, R., Garnham, P.C.C., Killick-Kendrick, R., and Bird, R. G. (1965). Nosematosis, a microsporidial infection of rodents and other animals, including man. Brit. Med. 2: 470–472.CrossRefGoogle Scholar
  57. Lewis, L. C., and Lynch, R. E. (197M. Lyophilization, vacuum drying, and subsequent storage of Nosema pyraustae spores. J. Invertebr. Pathol. 24: 109–153.Google Scholar
  58. Lillie, R. D. (1965). Histopathologic technic and practical histochemistry. McGraw-Hill Book Co., New York, Toronto, Sydney, London.Google Scholar
  59. Lom, J., and Vavra, J. (1962). Mucous envelopes of spores of the subphylum Cnidospora (Doflein 1901). Vest. Cs. Spol. Zool. 27: 4–6.Google Scholar
  60. Lom, J., and Vavra, J. (1963). The mode of sporoplasm extrusion in microsporidian spores. Acta. Protozool. 1: 81–89.Google Scholar
  61. Lom, J., and Weiser, J. (1969). Notes on two microsporidian species from Silurus glanis and on the systematic status of the genus Glugea Thelohan. Folia Parasit. (Praha) 16: 193–200.Google Scholar
  62. Lom, J., and Weiser, J. (1972). Surface pattern of some microsporidian spores as seen in the scanning electron microscope. Folia Parasitol. (Praha) 14: 359–363.Google Scholar
  63. Lotmar, R. (19M). Uber den Einfluss der Temperatur auf den Parasiten Nosema apis. Schweiz. Bienen.-Z. 67.: 17–19.Google Scholar
  64. Luft, J. H. (1971). Ruthenium red and violet. 1. chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat. Rec. 171: 307–368.Google Scholar
  65. Lynch, R. E., and Lewis, L. C. (1971). Reoccurrence of the microsporidan Perezia pyraustae in the European corn borer, Ostrinia nubilalis, reared on diet containing Fumidil B. J. Invertebr. Pathol. 17: 243–276.PubMedCrossRefGoogle Scholar
  66. Maddox, J. V. (1968). Generation time of the microsporidian, No8ema necatrix in larvae of the armyworm, Pseudaletia unipuncta. J. Invertebr. Pathol. 11: 90–96.CrossRefGoogle Scholar
  67. Martouret, D. (1962). Etude pathologiques sur le mode dfaction de Bacillus thuringiensis. Int. Congr. Ent., Twelth, Vienna, 1960. 2: 849–855.Google Scholar
  68. McLaughlin R. E. (1966). Laboratory techniques for rearing disease-free insect colonies: Elimination of Mattesia grandis McLaughlin, and Nosema sp. from colonies of boll weevils. J. Econ. Entomol. 59: 401–404.Google Scholar
  69. McLaughlin, R. E., Bell, M. R., and Daum, R. J. (1967). Suspension of microorganisms in a thixotropic solution. J. Insect. Pathol. 9: 35–39.Google Scholar
  70. McLaughlin, R. E., and Bell, M. R. (1970). Mass production in vivo of two protozoan pathogens, Mattesie grandis and Glngea gasti of the boll weevil, Anthonomus grandis. J. Invertebr. Pathol. 16: 84–88.CrossRefGoogle Scholar
  71. Michelson, E. H. (1963). Plistophore husseyiy sp. n., a microsporidian parasite of aquatic pulmonate snails. J. Invertebr. Pathol. 5: 28–38.Google Scholar
  72. Milner, R. J. (1972a). Nosema whiteiy a microsporidian pathogen of some species of Tribolium. 1. Morphology, life cycle, and generation time. J. Invertebr. Pathol. 19: 231–238.CrossRefGoogle Scholar
  73. Milner, R. J. (1972b). The survival of Nosema whitei spores stored at h°C. J. Invertebr. Pathol. 20: 256–257.CrossRefGoogle Scholar
  74. Montrey, R. D., Shadduck, J. A., and Pakes, S. P. (1973). In vitro study of host range of three isolates of Encephalitozoon (Nosema). J. Infect. Dis. 127: 150–159.CrossRefGoogle Scholar
  75. Nelson, J. B. (1962). An intracellular parasite resembling a microsporidian associated with ascites in Swiss mice. Proc. Soc. Exp. Biol. Med. 109: 711–717.CrossRefGoogle Scholar
  76. Nelson, J. B. (1967). Experimental transmission of a murine microsporidian in Swiss mice. J. Bacteriol. 91: 1340–1355.Google Scholar
  77. Nicholson, G. L., and Singer, S. J. (1971). Ferritin-conjugated plant agglutinins as specific saccharide stains for EM: application to saccharides bound to cell membranes. Proc. Nat. Acad. Sci. (U.S.) 68: 922–955.Google Scholar
  78. Nordin, G. L. (1971). Studies on a nuclear polyhedrosis virus and three species of microsporidia pathogenic to the fall webworm, Hyphantria cunea (Drury). Ph.D. thesis, Department of Entomology, University of Illinois, Urbana.Google Scholar
  79. Ohshima, K. (1937). On the function of the polar filament of Nosema bornbycis. Parasitology 29: 220–224.CrossRefGoogle Scholar
  80. Ormerod, W. E., Healey, P., and Armitage, P. (1963). A method of counting trypanosomes allowing simultaneous study of their morphology. Exp. Parasit. 13: 374–385.PubMedCrossRefGoogle Scholar
  81. Overstreet, R. M., and Weidner, E. (197*0. Differentiation of microsporidian sporetails in Inodosporus spraguei gen. et sp. n. Z. Parasitenk. 44: 169–186.Google Scholar
  82. Pakes, S. P., Shadduck, J. A., and Olsen, R. G. (1972). A diagnostic skin test for encephalitozoonosis (nosematosis) in rabbits. Lab. Anim. Sei. 22: 870–877.Google Scholar
  83. Percy, J. (1973). The intranuclear occurrence and fine structural details of schizonts of Perezia ftaniferanae (Microsporida: Nosematidae) in cells of Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). Canad. J. Zool. 51: 553–559.CrossRefGoogle Scholar
  84. Piekarski, G. (1937). Cytologische Untersuchungen an Paratyphus und Colibakterien. Arch. Mikrobiol. 8: 128–438.CrossRefGoogle Scholar
  85. Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope. J. Histochem. Cytochem. 15: 109–112.CrossRefGoogle Scholar
  86. Savage, K. E., and Lowe, R. E. (1970). Studies of Anopheles quadrimaculatus infected with a Nosema sp. Proc. Int. Colloq. on Insect Pathol., Fourth, College Park, Maryland.Google Scholar
  87. Sen Gupta, K. (196U). Cultivation of Nosema mesnili Paillot (Microsporidia) in vitro. Cur. Sei. 33: 107–108.Google Scholar
  88. Shadduck, J. A. (1969). Nosema curiculi: in vitro isolation. Science 166: 516–517.PubMedCrossRefGoogle Scholar
  89. Shortt, H. E., and Cooper, W. (1978). Staining of microscopical sections containing protozoal parasites by modification of McNamara’s method. Trans. R. Soc. Trop. Med. Hyg. 41 427–428.Google Scholar
  90. Smith, C. N. (ed.). (1966). “Insect Colonization and Mass Production.” Academic Press, New York.Google Scholar
  91. Southwood, T.R.E. (1966). Ecological methods with particular reference to the study of insect populations. London: Methuen.Google Scholar
  92. Sprague, Victor. (1965). Nosema sp. (Microsporidia, Nosematidae) in the musculature of the Crab, Callinectes sopidus. J. Protozool. 12: 66–70.CrossRefGoogle Scholar
  93. Sprague, V., Vernick, S. H., and Lloyd, B. J. Jr. (1968). The fine structure of Nosema sp. Sprague, 1965 (Microsporida, Nosematidae) with particular reference to stages in sporogony. J. Invertebr. Pathol. 12: 105–117.CrossRefGoogle Scholar
  94. Steche, W. (1965). Zur Ontologie von Nosema apis Zander in Mitteldarm der Arbeitsbiene. Bull, Apicole 8: 181–212.Google Scholar
  95. Undeen, A. H. (1975). Growth of Nosema algerae in pig kidney cell cultures. J. Protozool. 22: 107–110.PubMedCrossRefGoogle Scholar
  96. Undeen, A. H., and Alger, N. E. l97l). A density gradient method for fractionating microsporidian spores. J. Invertebr. Pathol. 18: 119–420.Google Scholar
  97. Undeen, A. H., and Maddox, J. V. (1973). The infection of non-mosquito hosts by injection with spores of the microsporidian Nosema algerae. J. Invertebr. Pathol. 22: 258–265.PubMedCrossRefGoogle Scholar
  98. Vavra, J. (1959). Beitrag zur Cytologie einiger Mikrosporidien. Vest. Cs. Zool. Spol. 23: 347–350.Google Scholar
  99. Vavra, J. (1963). Spore projections in Microsporidia. Acta Protozool. 1: 153–155.Google Scholar
  100. Vavra, J. (l96Ua). Recording microsporidian spores, J. Insect Pathol. 6: 258–260.Google Scholar
  101. Vavra, J. (l96Ub). Some recent advances in the study of microsporidian spores. Proc. Int. Congr. Parasitol., First, Roma, 1964, 1: 443–444Google Scholar
  102. Vavra, J. (1965). Etude au microscope électronique de la morphologie et du développement de quelques Microsporidies. C. R. Aead. Sei. Paris. 26l: 3467–3470.Google Scholar
  103. Vavra, J. (1972). Detection of polysaccharides in microsporidian spores by means of the periodic acid-thiosemicarbazide-silver proteinate test. J. Microscopie 4: 357–360.Google Scholar
  104. Vavra, J., and Undeen, A. H. (1970). Nosema algerae n. sp. (Cnidospora, Microsporida), a pathogen in a laboratory colony of Anopheles stephensi Liston (Diptera, Culicidae). J. Protozool. 17 240–249.PubMedCrossRefGoogle Scholar
  105. Vavra, J., Bedrnik, P., and Cinatl, J. (1972). Isolation and in vitro cultivation of the mammalian microsporidian Enoephalitozoon cuniculi. Folia parasit. (Praha) 19: 349–359.Google Scholar
  106. Vernick, S. H., Tousimis, A., and Sprague, V. (1969). Surface structure of the spores of Glugea weissenbergi. Proc. Annual EMSA. Twenty seventh.Google Scholar
  107. Walker, M. H., and Hinsch, O. W. (1972). Ultrastructural observations of a microsporidian protozoan parasite in Libinia dubia (Decapoda). I. Early spore development. Z. Parasitenk 39 17–26.CrossRefGoogle Scholar
  108. Weidner, E. (1970). Ultrastructural study of microsporidian development. I. Nosema sp. Sprague, 1965 in Callineotes sapidus Rathbun. Z. Zell forsch. 105: 33–51.CrossRefGoogle Scholar
  109. Weidner, E. (1972). Ultrastructural study of microsporidian invasion into cells. Z. Parasitenk. 40: 227–242.PubMedGoogle Scholar
  110. Weiser, J. (1961). Die Mikrosporidien als Parasiten der Insekten. Monographien zur angev. Entomol. Nr. 17. Verlag Paul Parey, Hamburg und Berlin. 149 pp.Google Scholar
  111. Weiser, J. (196U). Parasitology of black flies. Bull. W.H.O. 31: 483–485.Google Scholar
  112. Weiser, J. (1969). Immunity of insects to protozoa. In “Immunity to Parasitic Animals” ( G. J. Jackson, R. Herman, and I. Singer, eds.), 1: 129–147. Appleton-Century-Crofts, New York.Google Scholar
  113. Wright, R. D., and Lumsden, R. D. (1968). Ultrastructural and histochemical properties of the acanthocephalan epicuticle. J. Parasitol. 1: 1111–1123.CrossRefGoogle Scholar
  114. Yearian, W. C., Gilbert, K. L., and Warren, L. O. (1966). Rearing the fall webworm, Hyphantria ctmea (Lepidoptera: Arctiidae) on a wheat germ medium. J. Kons. Ent. Soc. 39: 195–199.Google Scholar

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© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Jiří Vávra
    • 1
  • J. V. Maddox
    • 2
  1. 1.Department of Protozoology Institute of ParasitologyCzechoslovak Academy of SciencesPraha 2, Vincino 7Czechoslovakia
  2. 2.Section of Economic EntomologyIllinois Natural History SurveyUrbanaUSA

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